Human tissue complementary DNA (cDNA) is DNA synthesized from a RNA template. Total RNA isolation is performed at our high performance facility by using modified guanidine thiocyanate techniques which ensure consistency. Our Total RNA sources originate from a wide variety of human tissues such as: human adult normal tissues, human diseased and tumor tissues, as well as, mouse, rat, monkey, and plant tissues.
Ready to use for PCR
Oligo dT primer used to ensure the presence of the entire 3′ end of cDNA
12 kb PCR amplicon had been successfully obtained with some templates indicating intact cDNAs
The largest selection of cDNAs from different tissues on the market
Documentation of tissues’ clinical histories available
Immediate PCR Amplification of known genes
Verification of genetic mutation
Comparison of a specific gene between different tissues
Analysis of mRNA alternative splicing
Gene cloning and target sequencing
The integrity of the RNA used for cDNA synthesis is examined by visual inspection for the presence of intact bands of 18s and 28s ribosomal RNA when electrophoreses on a denaturing agarose gel.
The quality and purity of total RNA were tested by spectrophotometer. A260/280 is between 1.8 and 2.0 (detected in 10 mM Tris-Cl, pH 7.5). The ratio of 28S/18S is ≥1.The RNA used for cDNA synthesis is treated by DNase I, and is tested as DNA free RNA by PCR.
The synthesized human, animal, and cell line cDNA was selected to ensure its full length. The cDNA was used as template for PCR amplification of ß-actin gene and an 838 bp ß-actin band was visualized on 1% agarose gel. ß-actin control primer is included. It is enough for 10 PCR reactions.
The synthesized plant tissue cDNA was used as template for PCR amplification of chloroplast gene. A 458 bp chloroplast band was visualized on 1% agarose gel. Chloroplast control primer is included. It is enough for 10 PCR reactions.