Reference RNA

At BioChain, we use proprietary isolation techniques to efficiently extract total RNA from tissues and cell lines, including difficult sample types such as cartilage. Our methods protect the RNA as it is being isolated and stored, so researchers can expect the highest integrity and quality possible for each sample type, with a wide range of downstream applications.

Features of Our Total RNA

  • Extensive quality control procedures to ensure high quality
  • High efficiency reverse transcription
  • Decontamination of polysaccharide, proteoglycan, RNase, and genomic DNA
  • Total RNA isolated from a wide variety of hard to obtain tissues
  • Custom services available upon request

Applications of Total RNA

  • cDNA synthesis and cDNA library construction
  • Purification of mRNA
  • MicroRNA study
  • Northern Blotting, RT-PCR, and RACE

Quality Control

Total RNA goes through rigorous quality control procedures to ensure the highest quality possible. Total RNA from normal, tumor, and diseased tissues and from different species are available, with detailed demographics and health information included such as clinical diagnosis and age. The integrity of the total RNAs is confirmed by checking for a ratio of greater than 1:1 between 28s and 18s ribosomal RNA.
BioChain provides consistent proof of the highest quality total RNA products. Our proprietary protocol results in products of high RIN value (Figure 1), and this can also be visualized using gel electrophoresis (Figure 2).
Figure 1. Total RNA from our human normal adrenal tissue with RIN value of 9.5.
Figure 2. Obtain consistently high quality total RNA using BioChain’s proprietary extraction methods.
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