At BioChain, matched total protein pairs are isolated by our proprietary techniques and stored in a protein storage buffer. This product is a useful tool for accelerating gene expression analysis by identifying tumor-specific and tumor metastatic proteins and genes.
BioChain's human cDNA matched pair products include:
Primary Tumor and Normal Pair (PP); genomic DNAs isolated from the primary tumor and its adjacent normal tissue
Primary and Metastatic Pair (PM); consists of genomic DNAs from the primary tumor and corresponding metastatic tumor
Custom extractions are available
Human tissue lysates isolated from tumor and normal tissues from the same patient
Decontamination of polysaccharide, proteoglycan, RNase, and genomic DNA
Extensive quality control procedures to ensure high quality
Documentation of tissues’ clinical history is available
Western Blot (10 µg to 20 µg per lane is recommended for a mini gel).
Enzymatic activity analysis
Tissue specific expression
Human tissue lysate is verified on SDS-PAGE analysis as a consistent pattern.
Human tissue lysate is positively confirmed by Western Blot using specific biomarkers:
GAPDH for total protein, cytoplasmic and universal total protein; Histone for nuclear protein; Na/K ATPase for membrane protein; Vimentin for cytoskeleton protein.