At BioChain, we use proprietary isolation techniques to efficiently extract human total RNA from tissues and cell lines, including difficult sample types such as cartilage. Our methods protect the RNA as it is being isolated and stored, so researchers can expect the highest integrity and quality possible for each sample type, with a wide range of downstream applications.
Features of Our Total RNA
Extensive quality control procedures to ensure high quality
High efficiency reverse transcription
Decontamination of polysaccharide, proteoglycan, RNase, and genomic DNA
Total RNA isolated from a wide variety of hard to obtain tissues
Custom services available upon request
Applications of Total RNA
cDNA synthesis and cDNA library construction
Purification of mRNA
Northern Blotting, RT-PCR, and RACE
Total RNA goes through rigorous quality control procedures to ensure the highest quality possible. Total RNA from normal, tumor, and diseased tissues and from different species are available, with detailed demographics and health information included such as clinical diagnosis and age. The integrity of the total RNAs is confirmed by checking for a ratio of greater than 1:1 between 28s and 18s ribosomal RNA.
BioChain provides consistent proof of the highest quality total RNA products. Our proprietary protocol results in products of high RIN value (Figure 1), and this can also be visualized using gel electrophoresis (Figure 2).
Figure 1. Total RNA from our human normal adrenal tissue with RIN value of 9.5.
Figure 2. Obtain consistently high quality total RNA using BioChain’s proprietary extraction methods.