Frequently Asked Questions

FAQs CATEGORY

FAQs PRODUCT TYPE

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Product
Do you provide CITES certificate for importing monkey related products?

Yes, CITES certificate can be provided with additional charges

Product
Do you provide custom made FISH probes?

Unfortunately, we do not provide this service at this time.

Product
How many matched pairs do you have for each tumor type?

We usually have more than 10 different donors.

Product
I would like to measure COX activity of mouse soleus muscle with this kit. Can you recommend a sample preparation (e.g. homogenate)?

Try the Mitochondria Isolation Kit.

Product
Can the microRNA extraction kit be used to isolate microRNA from mouse plasma? If so, what is the amount of plasma required and what is the average yield?

We have not tried this with the microRNA extraction kit. We believe the amount would be minimum and doubt you would get anything from the plasma.

Product
I did run qPCR with the Master Mix. However, no amplification was seen, why?

Please check your program settings. Also be sure to include positive and negative controls for troubleshooting purposes.

Product
What is EvaGreen and why do you have it in the qPCR mix instead of SYBR Green?

EvaGreen dye is a next-generation DNA-binding dye with features ideal for use in real-time PCR. Compare with SYBR Green, it has better dye properties such as less PCR inhibition, better stability and wider fluorescence spectra. The net results of application of EvaGreen dye in qPCR SuperMix are higher resolution melt curve (HRM) and lower Ct value at the same PCR condition.

Product
What is the advantage of chemically modified hotstart enzyme?

The chemically modified Taq enzyme can be activated partially or completely in a pre-PCR heat step, or can be activated slowly in a time-release manner during the denaturation steps of thermal cycling. With or without a limited up-front heat activation step, active enzyme is released slowly during thermal cycling to match template concentration and increase specificity. The chemical hotstart enzyme is also free of biological contamination due to the chemical modification method.

Product
What type of hotstart taq enzyme do you provide?

With licenced technology from Applied Biosciences, BioChain provides chemically modified form of Taq DNA polymerase which is in an inactive state, and by heat activation, the chemical modifier is permanently released, regenerating highly active enzyme.

Product
What is the reaction temperature for UltraScript RT?

The optimal fist-strand cDNA synthesis temperature for this enzyme is 60