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The FFPE fixation protocol has the following steps.
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Yes, CITES certificate can be provided with additional document charges and a minimum order of $3000. Please contact your sales representative for an estimate or email firstname.lastname@example.org.
Unfortunately, we do not provide this service at this time.
We usually have more than 10 different donors.
Try the Mitochondria Isolation Kit.
We have not tried this with the microRNA extraction kit. We believe the amount would be minimum and doubt you would get anything from the plasma.
Please check your program settings. Also be sure to include positive and negative controls for troubleshooting purposes.
EvaGreen dye is a next-generation DNA-binding dye with features ideal for use in real-time PCR. Compare with SYBR Green, it has better dye properties such as less PCR inhibition, better stability and wider fluorescence spectra. The net results of application of EvaGreen dye in qPCR SuperMix are higher resolution melt curve (HRM) and lower Ct value at the same PCR condition.
The chemically modified Taq enzyme can be activated partially or completely in a pre-PCR heat step, or can be activated slowly in a time-release manner during the denaturation steps of thermal cycling. With or without a limited up-front heat activation step, active enzyme is released slowly during thermal cycling to match template concentration and increase specificity. The chemical hotstart enzyme is also free of biological contamination due to the chemical modification method.