Frequently Asked Questions



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What is your FFPE fixation protocol?

The FFPE fixation protocol has the following steps.

  1. Fix the tissue in 10% formalin for 24 hours
  2. Ethanol gradient dehydration
  3. Use xylene for transparency
  4. Dip wax
  5. Embedding
General, How to Order, Terms and Conditions
Terms and Conditions

BioChain's Terms and Conditions can be found HERE.

Do you provide CITES certificate for importing monkey related products?

Yes, CITES certificate can be provided with additional document charges and a minimum order of $3000. Please contact your sales representative for an estimate or email

Do you provide custom made FISH probes?

Unfortunately, we do not provide this service at this time.

How many matched pairs do you have for each tumor type?

We usually have more than 10 different donors.

I would like to measure COX activity of mouse soleus muscle with this kit. Can you recommend a sample preparation (e.g. homogenate)?

Try the Mitochondria Isolation Kit.

Can the microRNA extraction kit be used to isolate microRNA from mouse plasma? If so, what is the amount of plasma required and what is the average yield?

We have not tried this with the microRNA extraction kit. We believe the amount would be minimum and doubt you would get anything from the plasma.

I did run qPCR with the Master Mix. However, no amplification was seen, why?

Please check your program settings. Also be sure to include positive and negative controls for troubleshooting purposes.

What is EvaGreen and why do you have it in the qPCR mix instead of SYBR Green?

EvaGreen dye is a next-generation DNA-binding dye with features ideal for use in real-time PCR. Compare with SYBR Green, it has better dye properties such as less PCR inhibition, better stability and wider fluorescence spectra. The net results of application of EvaGreen dye in qPCR SuperMix are higher resolution melt curve (HRM) and lower Ct value at the same PCR condition.

What is the advantage of chemically modified hotstart enzyme?

The chemically modified Taq enzyme can be activated partially or completely in a pre-PCR heat step, or can be activated slowly in a time-release manner during the denaturation steps of thermal cycling. With or without a limited up-front heat activation step, active enzyme is released slowly during thermal cycling to match template concentration and increase specificity. The chemical hotstart enzyme is also free of biological contamination due to the chemical modification method.