I got your total RNA, and I measured the DEPC water diluted total RNA concentration again by myself, but the concentration I got is lower than yours, can you tell me why?
I would suggest you use our method to measure the concentration:
Dilute the RNA in 10 mM Tris-Cl, pH 7.5 but not DEPC water. We usually took 2 ul RNA into 498 ul 10 mM Tris-Cl, (pH 7.5), then measure the OD, use the OD value x 40ug/ml, and then x250 for the final concentration. Using DEPC water will always result lower UV260/280 ratio and lower concentration, and the concentration measured by DEPC water is not stable.