cDNA

Human tissue complementary DNA (cDNA) is DNA synthesized from a RNA template. Total RNA isolation is performed at our high performance facility by using modified guanidine thiocyanate techniques which ensure consistency. Our Total RNA sources originate from a wide variety of human tissues such as: human adult normal tissues, human diseased and tumor tissues, as well as, mouse, rat, monkey, and plant tissues.

Features:

  • Ready to use for PCR
  • Oligo dT primer used to ensure the presence of the entire 3′ end of cDNA
  • 12 kb PCR amplicon had been successfully obtained with some templates indicating intact cDNAs
  • The largest selection of cDNAs from different tissues on the market
  • Documentation of tissues’ clinical histories available

Applications:

  • Immediate PCR Amplification of known genes
  • Verification of genetic mutation
  • Comparison of a specific gene between different tissues
  • Analysis of mRNA alternative splicing
  • Gene cloning and target sequencing

Quality Control

The integrity of the RNA used for cDNA synthesis is examined by visual inspection for the presence of intact bands of 18s and 28s ribosomal RNA when electrophoreses on a denaturing agarose gel.
The quality and purity of total RNA were tested by spectrophotometer. A260/280 is between 1.8 and 2.0 (detected in 10 mM Tris-Cl, pH 7.5). The ratio of 28S/18S is ≥1.The RNA used for cDNA synthesis is treated by DNase I, and is tested as DNA free RNA by PCR.
The synthesized human, animal, and cell line cDNA was selected to ensure its full length. The cDNA was used as template for PCR amplification of Ăź-actin gene and an 838 bp Ăź-actin band was visualized on 1% agarose gel. Ăź-actin control primer is included. It is enough for 10 PCR reactions.
The synthesized plant tissue cDNA was used as template for PCR amplification of chloroplast gene. A 458 bp chloroplast band was visualized on 1% agarose gel. Chloroplast control primer is included. It is enough for 10 PCR reactions.
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23 Results

Set Descending Direction
  1. 10 rxn
    C1234101-10
    B306113
    $237.00
  2. 40 rxn
    C1234226
    B802156
    $424.00
  3. 40 rxn
    C1234227
    C602016
    $424.00
  4. 40 rxn
    C1234230
    B312055
    $424.00
  5. 40 rxn
    C1236101Dia
    A809014
    $802.00
  6. 40 rxn
    C1236101Lcs
    $802.00
  7. 40 rxn
    C1236226Lcs
    B603127
    $802.00
  8. 40 rxn
    C1236226Lup
    A605168
    $811.00
  9. 40 rxn
    C1236227Dia
    A910119
    $802.00
  10. 40 rxn
    C1236227Lcs
    $802.00
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