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Total RNA

Features of Total RNA

Ribonucleic acid (RNA) is vital to the functioning of life. It is present in every cell in the body and has nearly limitless functions – from carrying genetic information to the production of deoxyribonucleic acid (DNA). RNA extraction is a process that must be handled with care due to the volatile presence of RNases. It is one of the most fragile and delicate samples to extract. RNases, which digest RNA upon contact, are present nearly everywhere, such as in water, skin, and sometimes even the researcher’s breath.

At BioChain, we use proprietary isolation techniques to efficiently extract total RNA from tissues and cell lines, including difficult sample types such as cartilage. Our methods protect the RNA as it is being isolated and stored, so researchers can expect the highest quality possible for each sample type, with maximum downstream applications. Contamination by RNase, genomic DNA polysaccharides, and proteoglycans are effectively eliminated using our methods. For example, RIN values of >8* are achievable using our extraction protocol. Our extraction techniques have also been optimized to collect RNA of a wide array of sizes, including small RNAs such as miRNA, siRNA, snRNA, and piRNA. These small RNAs have become of great interest in cancer research due to their ability to regulate gene expression. We include gel photos of the total RNA on our data sheets, which can generally be accessed online. RIN scores are also frequently listed so you can be certain of your starting material when you begin a new project. We have a comprehensive collection of normal, diseased, and tumor tissues from a wide variety of species to choose from. Whether you are studying Alzheimer’s disease in brain tissue, cirrhosis of the liver, or prostate cancer, BioChain likely has what you need. Tissues also come with demographic information.Save valuable research time and money by eliminating the hassle of locating and extracting RNA from a variety of sample types. Our procurement services can also help you find rare materials – enabling you to perform experiments that you thought were impossible.

Our Total RNA is available frozen and is shipped on dry ice. For international customers where saving on high shipping costs is a must, we also offer room temp RNA which is merely our total RNA that has been specially treated to be stable at ambient temperatures. Upon reconstitution, the product will be ready-to-use as total RNA.

*RIN values dependent on sample type

Applications of Total RNA

  • cDNA synthesis and cDNA library construction
  • Purification of mRNA
  • MicroRNA study
  • Northern Blotting, RT-PCR, and RACE

Advantages and Features of Our Total RNA

  • Extensive quality control procedures to ensure high quality
  • High efficiency reverse transcription
  • Decontamination of polysaccharide, proteoglycan, RNase, and genomic DNA
  • Total RNA isolated from a wide variety of hard to obtain tissues
  • Custom services available upon request

Quality Data

Total RNA goes through rigorous quality control procedures to ensure the highest quality possible. Total RNA from normal, tumor, and diseased tissues and from different species are available, with detailed demographics and health information included such as clinical diagnosis and age. The integrity of the total RNAs is confirmed by checking for a ratio of greater than 1:1 between 28s and 18s ribosomal RNA.

BioChain provides consistent proof of the highest quality total RNA products. Our proprietary protocol result in products of high RIN value (Figure 1), and this can also be visualized using gel electrophoresis (Figure 2).

Figure 1. Total RNA from our human normal adrenal tissue with RIN value of 9.5.

Figure 2. Obtain consistently high quality total RNA using BioChain’s proprietary extraction methods.

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