Matched Pair total RNA have been isolated from primary tumors and their adjacent unaffected normal tissue. This product is a useful tool for accelerating gene expression analysis by identifying tumor-specific genes and tumor metastatic genes.
Matched Pair total RNA isolated from tumor and adjacent normal tissues from the same patient.
Decontamination of polysaccharide, proteoglycan, RNase, and genomic DNA.
Extensive quality control procedures to ensure high quality.
High efficiency reverse transcription.
Northern Blotting, RT-PCR, and RACE
cDNA synthesis and cDNA library construction
cDNA probe for profiling study in gene expression
RNA protection and primer extension
Purification of mRNA
Next Generation RNA Sequencing
The integrity of the RNA is examined by visual inspection for the presence of intact bands of 18s and 28s ribosomal RNA when electrophoresis on a denaturing agarose gel
The quality and purity of total RNA were tested by spectrophotometer. A260/280 is between 1.8 and 2.1(detected in 10 mM Tris-HCl, pH 7.5).