Matched Pair Total Protein

At BioChain, matched total protein pairs are isolated by our proprietary techniques and stored in a protein storage buffer. This product is a useful tool for accelerating gene expression analysis by identifying tumor-specific and tumor metastatic proteins and genes.

BioChain's human Protein matched pair products include:

  • Primary Tumor and Normal Pair (PP); matched total protein isolated from the primary tumor and its adjacent normal tissue
  • Primary and Metastatic Pair (PM); consists of total protein from the primary tumor and corresponding metastatic tumor
  • Custom extractions are available


  • Human tissue lysates isolated from tumor and normal tissues from the same patient
  • Decontamination of polysaccharide, proteoglycan, RNase, and genomic DNA
  • Extensive quality control procedures to ensure high quality
  • Documentation of tissues’ clinical history is available


  • Western Blot (10 µg to 20 µg per lane is recommended for a mini gel).
  • Immunoprecipitation
  • Electrophoresis
  • Enzymatic activity analysis
  • Protein-protein interaction
  • Tissue specific expression

Quality Control

  • Human tissue lysate is verified on SDS-PAGE analysis as a consistent pattern.
  • Human tissue lysate is positively confirmed by Western Blot using specific biomarkers:
  • GAPDH for total protein, cytoplasmic and universal total protein; Histone for nuclear protein; Na/K ATPase for membrane protein; Vimentin for cytoskeleton protein.
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