Matched Pair RNA

Matched Pair total RNA have been isolated from primary tumors and their adjacent unaffected normal tissue. This product is a useful tool for accelerating gene expression analysis by identifying tumor-specific genes and tumor metastatic genes.

Features

  • Matched Pair total RNA isolated from tumor and adjacent normal tissues from the same patient.
  • Decontamination of polysaccharide, proteoglycan, RNase, and genomic DNA.
  • Extensive quality control procedures to ensure high quality.
  • High efficiency reverse transcription.

Applications

  • Northern Blotting, RT-PCR, and RACE
  • cDNA synthesis and cDNA library construction
  • cDNA probe for profiling study in gene expression
  • RNA protection and primer extension
  • RNA display
  • Purification of mRNA
  • Next Generation RNA Sequencing

Quality Control

  • The integrity of the RNA is examined by visual inspection for the presence of intact bands of 18s and 28s ribosomal RNA when electrophoresis on a denaturing agarose gel
  • The quality and purity of total RNA were tested by spectrophotometer. A260/280 is between 1.8 and 2.1(detected in 10 mM Tris-HCl, pH 7.5).
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