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Universal cDNA Reverse Transcribed by Random Hexamer: Rat Normal Tissues
C4434567-R
B706247
- Species: Rat
- Applications: NGS, qPCR
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Product Description
BioChain's universal cDNA is prepared using the following method. 10 µg total RNA was primed by an oligo dT primer or random hexamer through reverse transcription (RT) using MMLV reverse transcriptase in 40 µl final volume. The RT Reaction was stopped by heating at 65ºC for 10 minutes. The cDNA is in 1x RT buffer. (1x RT Buffer: 50 mM Tris-CI, pH 8.3, 75 mM KCI, 3 mM MgCI2, 10 mM DTT). The estimated cDNA concentration is about 2.5 ng/ul. 1 µ l cDNA is good enough for one PCR reaction.
Looking for multiple donors or additional samples?
Product Specifications
| Features | Specifications |
|---|---|
| Size | 100 rxn |
| Shipping | Dry Ice |
| Species | Rat |
| Race | Wistar |
| Sex | See DataSheet |
| Age At Sampling | See DataSheet |
| Diagnosis | Normal |
| Donor Status | Normal |
| Product Type | DNA |
| Applications | NGS, qPCR |
| Donor Information | Multiple Human Donors: Male: 5-7 wks old, 6 donors / Female: 5-7 wks old, 6 donors |
CATALOG #: C4434567-R
