Universal cDNA Reverse Transcribed by Random Hexamer: Dog Normal Tissues

C4734565-R B201117
  • Species: Dog
  • Applications: NGS, qPCR
BioChain's Dog Universal cDNA is prepared from major organs of both male and female Beagle dogs. This cDNA serves as a standard for comparison of gene expression by RT-PCR or as a gene pool for cloning dog genes.
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Product Description

BioChain's universal cDNA is prepared using the following method. 10 µg total RNA was primed by an oligo dT primer or random hexamer through reverse transcription (RT) using MMLV reverse transcriptase in 40 µl final volume. The RT Reaction was stopped by heating at 65ºC for 10 minutes. The cDNA is in 1x RT buffer. (1x RT Buffer: 50 mM Tris-CI, pH 8.3, 75 mM KCI, 3 mM MgCI2, 10 mM DTT). The estimated cDNA concentration is about 2.5 ng/ul. 1 µ l cDNA is good enough for one PCR reaction.
Looking for multiple donors or additional samples?

Product Specifications

Features Specifications
Size 100 rxn
Shipping Dry Ice
Species Dog
Race Beagle
Sex See DataSheet
Age At Sampling See DataSheet
Diagnosis Normal
Donor Status Normal
Product Type DNA
Applications NGS, qPCR
Donor Information Multiple Human Donors: Male: 1.5 yrs old, 1 donor / Female: 1.5 yrs old, 3 donors