Universal cDNA Reverse Transcribed by Oligo dT Primer: Rat Normal Tissues

C4434567 B706249
  • Species: Rat
  • Applications: NGS, qPCR
BioChains' Rat Universal cDNA is prepared from several male and female Sprague Dawley or Wistar rat whole bodies without fur. This cDNA serves as a standard for comparison of gene expression by RT-PCR or as a gene pool for cloning rat genes.
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Product Description

BioChain's universal cDNA is prepared using the following method. 10 µg total RNA was primed by an oligo dT primer or random hexamer through reverse transcription (RT) using MMLV reverse transcriptase in 40 µl final volume. The RT Reaction was stopped by heating at 65ºC for 10 minutes. The cDNA is in 1x RT buffer. (1x RT Buffer: 50 mM Tris-CI, pH 8.3, 75 mM KCI, 3 mM MgCI2, 10 mM DTT). The estimated cDNA concentration is about 2.5 ng/ul. 1 µ l cDNA is good enough for one PCR reaction.
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Product Specifications

Features Specifications
Size 100 rxn
Shipping Dry Ice
Species Rat
Race Wistar
Sex See DataSheet
Age At Sampling See DataSheet
Diagnosis Normal
Donor Status Normal
Product Type DNA
Applications NGS, qPCR
Donor Information Multiple Human Donors: Male: 5-7 wks old, 6 donors / Female: 5-7 wks old, 6 donors