cDNA - Liver Cirrhosis: Spleen

C1236246Lcs C201152
  • Species: Human
  • Disease: Liver Cirrhosis
  • Applications: NGS, qPCR
BioChain's PCR Ready First Strand cDNAs are available from human liver diseased tissues. 1 µl cDNA is sufficient for each PCR reaction.
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Product Description

First-Strand cDNA is synthesized from RNA isolated from a wide variety of documented human adult normal tissues, human diseased and tumor tissues, mouse, rat, monkey and plant tissues. Total RNA used for cDNA synthesis is isolated by modified guanidine thiocyanate techniques. 10 µg total RNA was primed by an oligo dT primer and reverse transcription (RT) using MMLV reverse transcriptase in 40 µl final volume. RT reaction was stopped by heating at 65°C for 10 minutes. The cDNA is delivered in 1x RT buffer (1x RT Buffer: 50 mM Tris-Cl, pH 8.3, 75 mM KCl, 3 mM MgCl2, 10 mM DTT). 1 µl cDNA is sufficient for one PCR reaction. The 5′ end of human clathrin cDNA (a 6 kb gene) has been amplified by PCR from all of these cDNAs.
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Product Specifications

Features Specifications
Size 40 rxn
Tissue Name Spleen
Shipping Dry Ice
Species Human
Sex Male
Age At Sampling 70 yrs
Tissue Spleen
Diagnosis Liver Cirrhosis
Donor Status Disease
Product Type DNA
Disease Liver Cirrhosis
Applications NGS, qPCR
Donor Information Male: 70 yrs old, 1 donor