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cDNA - Human Adult Normal Tissue: Brain: Cerebellum (right)
C1234041-10
C201117
- Species: Human
- Applications: NGS, qPCR
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Product Description
First-Strand cDNA is synthesized from RNA isolated from a wide variety of documented human adult normal tissues, human diseased and tumor tissues, mouse, rat, monkey and plant tissues. Total RNA used for cDNA synthesis is isolated by modified guanidine thiocyanate techniques. 10 µg total RNA was primed by an oligo dT primer and reverse transcription (RT) using MMLV reverse transcriptase in 40 µl final volume. RT reaction was stopped by heating at 65°C for 10 minutes. The cDNA is delivered in 1x RT buffer (1x RT Buffer: 50 mM Tris-Cl, pH 8.3, 75 mM KCl, 3 mM MgCl2, 10 mM DTT). 1 µl cDNA is sufficient for one PCR reaction. The 5′ end of human clathrin cDNA (a 6 kb gene) has been amplified by PCR from all of these cDNAs.
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Product Specifications
| Features | Specifications |
|---|---|
| Size | 10 rxn |
| Tissue Name | Brain: Cerebellum (Right) |
| Shipping | Dry Ice |
| Species | Human |
| Race | Asian |
| Sex | Male |
| Age At Sampling | 33 yrs |
| Tissue 2 | Cerebellum (right) |
| Tissue | Brain |
| Diagnosis | Normal |
| Donor Status | Normal |
| Product Type | DNA |
| Applications | NGS, qPCR |
| Donor Information | Male: 33 yrs old, 1 donor |
CATALOG #: C1234041-10
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Application Notes
Data Sheet
SDS
Certificate of Analysis
Test
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Sample Work Flow
Scalability of vamPure
gDNA was extracted from either 200 µl or 1 mL of input blood using vamPure. The resulting yields show that the kit is highly scalable.