cDNA - Human Adult Normal Tissue: Brain: Cerebellum (right)

C1234041-10 C201117
  • Species: Human
  • Applications: NGS, qPCR
BioChain's PCR Ready First Strand cDNAs are available from different human adult normal tissues. 1 µl cDNA is sufficient for each PCR reaction.
$195.00
Need help with your order?
Find out more about placing an order here or contact us for technical support.
Pricing is for US customers only. For international pricing, please contact your local distributor. View Distributors Here.

Product Description

First-Strand cDNA is synthesized from RNA isolated from a wide variety of documented human adult normal tissues, human diseased and tumor tissues, mouse, rat, monkey and plant tissues. Total RNA used for cDNA synthesis is isolated by modified guanidine thiocyanate techniques. 10 µg total RNA was primed by an oligo dT primer and reverse transcription (RT) using MMLV reverse transcriptase in 40 µl final volume. RT reaction was stopped by heating at 65°C for 10 minutes. The cDNA is delivered in 1x RT buffer (1x RT Buffer: 50 mM Tris-Cl, pH 8.3, 75 mM KCl, 3 mM MgCl2, 10 mM DTT). 1 µl cDNA is sufficient for one PCR reaction. The 5′ end of human clathrin cDNA (a 6 kb gene) has been amplified by PCR from all of these cDNAs.
Looking for multiple donors or additional samples?

Product Specifications

Features Specifications
Size 10 rxn
Tissue Name Brain: Cerebellum (Right)
Shipping Dry Ice
Species Human
Race Asian
Sex Male
Age At Sampling 33 yrs
Tissue 2 Cerebellum (right)
Tissue Brain
Diagnosis Normal
Donor Status Normal
Product Type DNA
Applications NGS, qPCR
Donor Information Male: 33 yrs old, 1 donor

Test

Test 

Test

Sample Work Flow

Scalability of vamPure

gDNA was extracted from either 200 µl or 1 mL of input blood using vamPure. The resulting yields show that the kit is highly scalable.