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|Tool Type||Sample Prep Kits|
Extract high quality cell free DNA from plasma, serum, and urine for Next Generation Sequencing (NGS), Bisulfite Sequencing, PCR, qPCR, ddPCR and other demanding applications.
Versatile: Extraction from <1mL to >10mL serum or plasma – in single tubes or 24- to 96-well plates
Automation Friendly: On both the KingFisher™ and liquid handling instruments, with extractions taking approximately 50 minutes
Time Efficient: Manual extraction protocol takes less than 75 minutes
Cost Effective: Competitive price <$3 per extraction from 1 mL plasma (List price for 250mL kit)
Elution volumes: Range from 15 µL to 50 µL
The cfPure® protocol is rapid and easy, allowing users to process several samples in an hour or less and making it ideal for biomarker screening. BioChain has developed its own silica-coated magnetic bead technology, which is designed to efficiently recover low molecular weight cell free DNA during isolation. In addition to being easy to perform, and easy to automate, you can use less magnetic bead solution if you are purifying small amounts of plasma – saving you money. The key to successful analysis of cfDNA is to ensure efficient purification of smaller DNA fragments (100-500 bp). As Figure 1 shows, the cfPure® Cell Free DNA Extraction Kit does efficiently recover these important small fragments.
Cfpure is designed to be a scalable kit (Fig. 2.). This means that you can use just the amount of beads and reagents that you need for your cfDNA extraction. This also means that the number of extractions per kit is dependent on the amount of sample you wish to purify DNA from. For example, if you are working with 1mL samples, our 100mL kit (Catalog # K5011610) can process up to 100 samples, and our 250 mL kit (Catalog # K5011610) can process 250 samples. If you are working with 10mL samples, our 100mL kit (Catalog # K5011610) can process up to 10 samples, and our 250 mL cell free DNA Extraction kit (Catalog # K5011625) can process 25 samples.