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BioChain's Alpha-Glucosidase Assay Kits are designed to measure both alpha and beta glucosidase activity directly in biological samples without pretreatment. The improved method utilizes p-nitrophenyl-alpha-Dglucopyranoside that is hydrolyzed specifically by alpha-glucosidase or p-nitrophenyl-a-Dglucopyranoside that is hydrolyzed specifically by a-glucosidase into a yellow colored product (maximal absorbance at 405 nm). The rate of the reaction is directly proportional to the enzyme activity.
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