Eva QPCR SuperMix Kit

Overview

BioChain’s QPCR SuperMix is a 2x concentration of premix reagent including Hotstart DNA polymerase and specially formulated real time buffer designed for real-time PCR with intercalator format. Eva and SYBR Green QPCR Supermix is in double-stranded cDNA detection format, while Pro QPCR Supermix is in the format of fluorescent probe-based detection assays, such as TaqMan or molecular beacons.

Description

Polymerase Chain Reaction (PCR) has become one of the most important technologies in the scientific community since its introduction in 1985. BioChain provides the most common used qPCR and PCR enzyme and reagents, such as qPCR SuperMix, Taq polymerase, dNTP, primers, at the most competitive price. This product line offers our customers of genomic DNA, RNA, and cDNA, a convenient and economic source for large quantity PCR enzymes and reagents.

    • Protective Role of Smad6 in Inflammation‐Induced Valvular Cell Calcification JJ Lim, X Li, J Lu, TM Pedego… – Journal of Cellular …, 2015– Wiley Online Library  Received 22 January 2015; Revised 31 March 2015; Accepted 1 April 2015  Song et al., 2015), and inhibition of BMP-2 signaling causes reduced vascular calcification (Derwall et al.,  performed using gene specific primers, One-Step qRT-PCR SuperMix Kit (BioChain) and 
    • SIRT3 reverses aging-associated degeneration Katharine Brown, Stephanie Xie, Xiaolei Qiu, Mary Mohrin, Jiyung Shin, Yufei Liu, Dan Zhang, David T Scadden, Danica Chen Cell Rep 0, 2013-02-21, 3, 319-27 … Reverse transcription was performed using qScript cDNA SuperMix (Quanta BioSciences). Gene expression was determined by real-time PCR using Eva qPCR SuperMix kit (BioChain Institute) on an ABI StepOnePlus. GAPDH or β-actin was used as an internal control. …
    • Presynaptic modulation by somatostatin in the rat neostriatum is altered in a model of parkinsonism Violeta G López-Huerta, Eduardo Blanco-Hernández, José Bargas, Elvira Galarraga Neurophysiol. (3.1), 2012-08-16, 108, 1032-43 … 5′-AGGTCAATGAAGGGGTCGTTG-3′ (PCR product: 103 bp). The Eva qPCR Super mix kit (BioChain, Hayward, CA) was used for real-time PCR with the manufacturer’s specifications. Thermal cycling included 40 cycles of …
    • Expression of lactoperoxidase in differentiated mouse colon epithelial cells Byung-Wook Kim, R Steven Esworthy, Maria A Hahn, Gerd P Pfeifer, Fong-Fong Chu Free Radic. Biol. Med. (5.7), 2012-05-01, 52, 1569-76 … 3′. The mRNA levels were normalized against β-actin mRNA (5′-GCTCCTCCTGAGCGCAAGT- 3′ and 5′-TCATCGTACTCCTGCTTGCTGAT-3′). All qPCRs were performed with the Eva qPCR SuperMix kit containing SYBR green dye (BioChainInstitute, Hayward, CA …
    • Angiopoietin-like 4 (Angptl4) protein is a physiological mediator of intracellular lipolysis in murine adipocytes Nora E Gray, Lily N Lam, Karen Yang, Anna Y Zhou, Suneil Koliwad, Jen-Chywan Wang Biol. Chem. (5.3), 2012-03-09, 287, 8444-56 … Reverse transcription was performed as described (6). The resulting cDNA was diluted to 170 μl, and 3.5 μl was used to perform qPCR in a 25-μl reaction using the EVA qPCR SuperMix kit (BioChain) per the manufacturer’s protocol. …
    • Yifan Geng, Jeffrey J. Hsu, Jinxiu Lu, Tabitha C. Ting, Makoto Miyazaki, Linda L. Demer, and Yin Tintut Role of Cellular Cholesterol Metabolism in Vascular Cell Calcification Biol. Chem., Sep 2011; 286: 33701 – 33706. … …from cells using TRIzol reagent (Invitrogen). Real-time RT-quantitative PCR (qPCR) was performed using a One-Step qRT-PCR kit (BioChainInstitute, Inc. Hayward, CA) in an Mx3005P system (Stratagene La Jolla, CA). beta-Actin was used for normalization. Data Analysis… …
    • Wendy Tseng, Lucia S. Graham, Yifan Geng, Aneela Reddy, Jinxiu Lu, Rita B. Effros, Linda Demer, and Yin Tintut PKA-induced Receptor Activator of NF- B Ligand (RANKL) Expression in Vascular Cells Mediates Osteoclastogenesis but Not Matrix Calcification Biol. Chem., Sep 2010; 285: 29925 – 29931. … …Total RNA was isolated using TRIzol reagent (Invitrogen). Real-time PCR was performed using the One-Step qRT-PCR SuperMix Kit (BioChain, Inc.) and Mx3005P (Stratagene). Western Analysis Western analysis of whole cell lysates was performed using standard protocols… …
    • Peng Jin, Xiao-juan Lu, Jian-qiu Sheng, Lei Fu, Xiao-ming Meng, Xin Wang, Tai-ping Shi, Shi-rong Li, and Jianyu Rao Estrogen Stimulates the Expression of Mismatch Repair Gene hMLH1in Colonic Epithelial Cells Cancer Prevention Research, Aug 2010; 3: 910 – 916. … …the results of semiquantitative RT-PCR in COLO205 cells. PCR was done in a volume of 20 muL containing 12.5 muL 2 PCR Mix (BioChain), 2 muL cDNA, 0.3 muL EvaGreen (Biotium), 0.5 muL of primers each, and 4.2 muL DEPC-treated water. The mixtures were amplified… …
    • Wendy Tseng, Jinxiu Lu, Gail A. Bishop, Andrew D. Watson, Andrew P. Sage, Linda Demer, and Yin Tintut Regulation of interleukin-6 expression in osteoblasts by oxidized phospholipids J. Lipid Res., May 2010; 51: 1010 – 1016. … …Total RNA was isolated using TRIzol reagent (Invitrogen). Real-time PCR was performed using the One-Step qRT-PCR SuperMix Kit (BioChain, Inc.) and Mx3005P (Stratagene). DNA constructs Generation of murine IL-6 promoter luciferase reporter constructs was
    • Suneil K. Koliwad, Taiyi Kuo, Lauren E. Shipp, Nora E. Gray, Fredrik Backhed, Alex Yick-Lun So, Robert V. Farese, Jr, and Jen-Chywan Wang Angiopoietin-like 4 (ANGPTL4, Fasting-induced Adipose Factor) Is a Direct Glucocorticoid Receptor Target and Participates in Glucocorticoid-regulated Triglyceride Metabolism Biol. Chem., Sep 2009; 284: 25593 – 25601. … …dNTP (Invitrogen), 0.2 SYBR Green I dye (Molecular Probes), and 250 nm of each primer. Alternatively, EVA QPCR SuperMix Kit (Biochain) was used per the manufacturer’s protocol. qPCR was performed in either an Opticon-2 DNA Engine (MJ Research) or a 7900HT PCR… …
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Certificate of Analysis
Product Manual
  • K5052200
  • 200 rxn