Bilirubin Assay Kit
BioChain’s bilirubin assay kit is a simple, direct and automation-ready assay for measuring bilirubin concentration directly in blood specimen in 96-well or cuvette formats. The improved Jendrassik-Grof method utilizes the reaction of bilirubin with diazotized sulfanilic acid, in which a red colored product is formed. The intensity of the color, measured at 510-550nm, is an accurate measure of the bilirubin level in the sample. Total bilirubin is assessed using caffeine benzoate to split bilirubin from the unconjugated bilirubin protein complex.
BioChain’s BioAssay kits are for both routine laboratory tests and high-throughput drug screening applications. These kits use harmless non-radioactive assay formats such as fluorescence, bio- and chemiluminescence detection techniques. With our assay kits, researchers need little to no time for assay optimization.