At BioChain, we use proprietary isolation techniques to efficiently extract total RNA from tissues, including difficult sample types such as cartilage. Our methods protect the RNA as it is being isolated and stored, so you can expect the highest quality possible for each sample typ. For example, RIN values of >8* are achievable using our extraction protocol.
Our extraction techniques have also been optimized to collect RNA of a wide array of sizes, including small RNAs such as miRNA, siRNA, snRNA, and piRNA. To confirm the presence of the desired RNA size classes in each lot of material, we include gel photos on our datasheets, which you can generally access online. RIN scores are frequently listed as well, or are available on request, so you can be certain of your starting material when you begin a new project.
We have a comprehensive collection of normal, diseased, and tumor tissues from a wide variety of species to choose from. We also offer custom procurement and collection services, so that you can be sure to get exactly the RNA extracts that you need – including RNA from non-mammalian animals and plants.
Therefore, we can save you valuable research time and money by eliminating the hassle of locating and extracting RNA from a variety of sample types. Our procurement services can also help you find rare materials – enabling you to perform experiments that you thought were impossible.
*RIN values dependent on sample type
Applications of Total RNA
- cDNA synthesis and cDNA library construction
- Purification of mRNA
- MicroRNA study
- Northern Blotting, RT-PCR, and RACE
Advantages and Features of Our Total RNA
- Extensive quality control procedures to ensure high quality
- High efficiency reverse transcription
- Decontamination of polysaccharide, proteoglycan, RNase, and genomic DNA
- Total RNA isolated from a wide variety of hard to obtain tissues
Let BioChain provide you with the highest quality total RNA products. Our strict quality control procedures consistently result in products of high RIN value (Figure 1), and this can also be visualized using gel electrophoresis (Figure 2).
Figure 1. Total RNA from our human normal adrenal tissue with RIN value of 9.5.
Figure 2. Obtain consistently high quality total RNA using BioChain’s proprietary extraction methods.