Total Protein Extraction Kit

K3011010
  • Applications: Extraction, Western Blot
BioChain's Total Protein Extraction Kit is designed for rapid and efficient isolation of total protein tissue lysates or cell lysates. The protein extracts isolated using this kit are native and are ready-to-use for many downstream applications.
Grouped product items
Product Name Qty
Total Protein Extraction Kit: TM Buffer
$117.00
Total Protein Extraction Kit: 50X PI
$249.00
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Product Description

BioChain’s Total Protein Extraction Kit is designed for rapid and efficient isolation of total protein lysates from virtually any tissue or cell sample. The system utilizes our proprietary buffer that has been optimized to prepare the highest quality total protein extractions. The kit also includes our proprietary protease inhibitor cocktail to prevent protein degradation. In conjunction, these two components result in protein extracts that are native and may be used for downstream applications such as SDS-PAGE, isoelectric focusing and 2-D gel electrophoresis, Western blotting, tissue-specific protein expression analysis, immunoprecipitation, protein-protein interaction studies, gel mobility shift analysis, DNA-protein interaction studies, antibody production, and enzymatic activity assays.
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Product Specifications

Features Specifications
Size 1 kit
Shipping Dry Ice
Sex Contact Us
Age At Sampling Contact Us
Applications Extraction, Western Blot
Sample Type Cell, Tissue
Features • Versatile: Extraction from as little as 0.1 g of tissue or 2.5 million cells and up to 5 g of tissue or 125 million cells • Time Efficient: Manual extraction protocol takes roughly one hour • Cost Effective: Competitive price <$7 per extraction from 100 mg of tissue

High Quality Total Protein Tissue Lysates

Using BioChain’s Total Protein Extraction Kit results in high-quality proteins that show an absence of smearing on an SDS-PAGE gel; extraction of total proteins is also demonstrated by the range in size from 10 kd to greater than 200 kd (Figure 1).

 

Figure 1. 50 μg of total proteins resolved on a 4-20% gradient SDS-PAGE gel (top) and expression of GAPDH* was verified using a Western blot (bottom). Total proteins were isolated from 16 different human tissues using the Total Protein Extraction kit as follows:

Lane 1: Placenta                  Lane 2: Testis                  

Lane 3: Prostate              Lane 4: Uterus

Lane 5: Rectum                  Lane 6: Colon                    

Lane 7: Small Intestine    Lane 8: Stomach

Lane 9: Skeletal Muscle     Lane 10: Spleen                 

Lane11: Pancreas            Lane 12: Lung

Lane 13: Liver                     Lane14: Kidney                 

Lane15: Brain                   Lane 16: Heart

*GAPDH expression levels dependent on tissue type