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Total Protein Extraction Kit
K3011010
- Applications: Extraction, Western Blot
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Product Description
BioChain’s Total Protein Extraction Kit is designed for rapid and efficient isolation of total protein lysates from virtually any tissue or cell sample. The system utilizes our proprietary buffer that has been optimized to prepare the highest quality total protein extractions. The kit also includes our proprietary protease inhibitor cocktail to prevent protein degradation. In conjunction, these two components result in protein extracts that are native and may be used for downstream applications such as SDS-PAGE, isoelectric focusing and 2-D gel electrophoresis, Western blotting, tissue-specific protein expression analysis, immunoprecipitation, protein-protein interaction studies, gel mobility shift analysis, DNA-protein interaction studies, antibody production, and enzymatic activity assays.
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Product Specifications
| Features | Specifications |
|---|---|
| Size | 1 kit |
| Shipping | Dry Ice |
| Sex | Contact Us |
| Age At Sampling | Contact Us |
| Applications | Extraction, Western Blot |
| Sample Type | Cell, Tissue |
| Features | • Versatile: Extraction from as little as 0.1 g of tissue or 2.5 million cells and up to 5 g of tissue or 125 million cells • Time Efficient: Manual extraction protocol takes roughly one hour • Cost Effective: Competitive price <$7 per extraction from 100 mg of tissue |
CATALOG #: K3011010
Protocols
Flyers & Brochures
SDS
Certificate of Analysis
High Quality Total Protein Tissue Lysates
Using BioChain’s Total Protein Extraction Kit results in high-quality proteins that show an absence of smearing on an SDS-PAGE gel; extraction of total proteins is also demonstrated by the range in size from 10 kd to greater than 200 kd (Figure 1).
Figure 1. 50 μg of total proteins resolved on a 4-20% gradient SDS-PAGE gel (top) and expression of GAPDH* was verified using a Western blot (bottom). Total proteins were isolated from 16 different human tissues using the Total Protein Extraction kit as follows:
Lane 1: Placenta Lane 2: Testis
Lane 3: Prostate Lane 4: Uterus
Lane 5: Rectum Lane 6: Colon
Lane 7: Small Intestine Lane 8: Stomach
Lane 9: Skeletal Muscle Lane 10: Spleen
Lane11: Pancreas Lane 12: Lung
Lane 13: Liver Lane14: Kidney
Lane15: Brain Lane 16: Heart
*GAPDH expression levels dependent on tissue type
