Reduction of intestinal mucosal immune function in heat-stressed rats and bacterial translocation
Xiaoxi Liu, Huanrong Li, An Lu, Yougang Zhong, Xiaolin Hou, Ning Wang, Dan Jia, Junlan Zan, Hong Zhao, Jianqin Xu, Fenghua Liu
Int J Hyperthermia (2.9), 2012-11-16, 28, 756-65
… Thus, protein from rat jejunum was extracted by a total protein extraction kit (K3011010, BioChain, San Francisco, CA, USA). The concentration of extracted protein was quantified by a BCA protein assay kit (23225, Pierce, Thermo Scientific, Rockford, IL, USA). …
Carnosic acid (CA) prevents lipid accumulation in hepatocytes through the EGFR/MAPK pathway
Ting Wang, Yasuhiro Takikawa, Takahito Tabuchi, Takumi Satoh, Kunio Kosaka, Kazuyuki Suzuki
J. Gastroenterol. (3.6), 2012-07-19, 47, 805-13
… tractant protein 1 (MCP-1). Western blot analysis Total protein was isolated from liver tissue or HepG2 cells by a total protein extraction kit purchased from BioChain Institute, Inc. (Hayward, CA, USA). The nuclear fraction was …
Ali Ö. Yildirim, Vandana Muyal, Gerrit John, Bernd Müller, Carola Seifart, Michael Kasper, and Heinz Fehrenbach Palifermin Induces Alveolar Maintenance Programs in Emphysematous Mice Am. J. Respir. Crit. Care Med., Apr 2010; 181: 705 – 717.
… …details.) Protein Isolation and Immunoblotting Protein was extracted from lung tissue using total protein extraction kit (Biochain Institute, Hayward, CA). Protein concentrations were measured using BCA protein assay kit (Pierce, Rockford, IL). After fragmentation… …
Ali O Yildirim, Vandana Muyal, Gerrit John, Bernd Mueller, Carola Seifart, Michael Kasper, and Heinz Fehrenbach Palifermin Induces Alveolar Maintenance Programs in Emphysematous Mice J. Respir. Crit. Care Med., Dec 2009; 10.1164/rccm.200804-573OC.
… …extracted from lung tissue using total protein extraction kit (BiochainInstitute, Hayward, USA). Protein concentrations were measured using BCATM protein assay kit (Pierce, Rockford, USA). After fragmentation…extracted from lung tissue using total protein extraction kit (Biochain Institute, Hayward, USA) according to the manufacturer’s protocol… …
Emily I. Chen, Johannes Hewel, Joseph S. Krueger, Claire Tiraby, Martin R. Weber, Anastasia Kralli, Katja Becker, John R. Yates, III, and Brunhilde Felding-Habermann Adaptation of Energy Metabolism in Breast Cancer Brain Metastases
Cancer Res., Feb 2007; 67: 1472 – 1486.
… …extracts were prepared with the TotalProteinExtraction kit (BioChain, Hayward, CA) and protein concentration was determined…extracts were prepared with the TotalProteinExtraction kit (BioChain) and protein concentrations determined by BCA assay (Pierce… … link
Pinthus JH, Bryskin I, Trachtenberg J, Lu JP, Singh G, Fridman E, Wilson BC. Androgen Induces Adaptation to Oxidative Stress in Prostate Cancer: Implications for Treatment with Radiation Therapy.
2007 Jan; 9(1): 68-80.
… …Centrifuging at 12,000g for 15 minutes separated insoluble materials. Supernatants were dissolved in Laemmli sample buffer. Protein purification from tumor tissues was performed using a protein extraction kit (Biochain Institute, Inc., Greenland, NH)…. … Ref. link
Jia-Perng Chen, Hsin-Lin Lu, Szu-Liang Lai, Gabriele S. Campanella, Jui-Ming Sung, Mei-Yi Lu, Betty A. Wu-Hsieh, Yi-Ling Lin, Thomas E. Lane, Andrew D. Luster, and Fang Liao Dengue Virus Induces Expression of CXC Chemokine Ligand 10/IFN–Inducible Protein 10, Which Competitively Inhibits Viral Binding to Cell Surface Heparan Sulfate
Immunol., Sep 2006; 177: 3185 – 3192.
… …the reference were approximately equal. ELISA Livers were weighed and homogenized on ice in protein extraction buffer (BioChainInstitute) using a tapered tissue grinder (Wheaton Science Products). The resultant homogenates were centrifuged at 15,000… … Ref. link
Chang, R. Yamada, A. Suzuki, Y. Kochi, T. Sawada, and K. Yamamoto Citrullination of fibronectin in rheumatoid arthritis synovial tissue
Rheumatology, Nov 2005; 44: 1374 – 1382.
… …the Total Protein Extraction Kit (Biochain, USA) according to the manufacturers…prostate and thymus adenocarcinoma (BioChain). Fn in the total proteins was precipitated…the Total Protein Extraction Kit (Biochain, USA) according to the manufacturer’s… …
Emily I. Chen, Laurence Florens, Fumiko T. Axelrod, Edward Monosov, Carlos F. Barbas III, John R. Yates III, Brunhilde Felding-Habermann, and Jeffrey W. Smith Maspin alters the carcinoma proteome
FASEB J, Apr 2005; 10.1096/fj.04-2970fje.
… …using TotalProteinExtraction kit (BioChain, Hayward, CA), and protein concentration…homogenized in the lysis buffer from the Biochain total protein extraction kit (Biochain). Cellular 20S proteasome activity… …
Chang, R. Yamada, A. Suzuki, T. Sawada, S. Yoshino, S. Tokuhiro, and K. Yamamoto Localization of peptidylarginine deiminase 4 (PADI4) and citrullinated protein in synovial tissue of rheumatoid arthritis
Rheumatology, Jan 2005; 44: 40 – 50.
… we purified total protein of RA synovial tissues and leucocytes using total protein extraction kits (Biochain). …