- Cerium oxide nanoparticles induce cytotoxicity in human hepatoma SMMC-7721 cells via oxidative stress and the activation of MAPK signaling pathways
Guilin Cheng, Wei Guo, Lu Han, Erlei Chen, Lingfang Kong, Lili Wang, Wenchao Ai, Naining Song, Haishan Li, Huiming Chen
Toxicol In Vitro (2.5), 2013-04-25, 27, 1082-8
… 2.6. Western blot analyses. Protein was extracted from SMMC-7721 cells using a total protein extraction kit (BioChain, Hayward, CA, USA) after each treatment; the concentration was determined using a bicinchoninic acid (BCA) protein assay (Pierce, Rockford, IL, USA). …
- Screening citrullinated proteins in synovial tissues of rheumatoid arthritis using 2-dimensional western blotting
Xiaotian Chang, Yan Zhao, Yuejian Wang, Yu Chen, Xinfeng Yan
Rheumatol. (3.6), 2013-03-04, 40, 219-27
… the sample loading. Immunoprecipitation. Total protein was purified from the synovial tissues of patients with RA (n = 5) using the Total Protein Extraction kit (BioChain) according to the manufacturer’s instructions. A1AT, keratin …
- Involvement of oxidative stress and mitogen-activated protein kinase signaling pathways in heat stress-induced injury in the rat small intestine
Jin Yu, Fenghua Liu, Peng Yin, Hong Zhao, Weili Luan, Xiaolin Hou, Yougang Zhong, Dan Jia, Junlan Zan, Wuren Ma, Banchao Shu, Jianqin Xu
Stress (2.6), 2013-01-11, 16, 99-113
… Western-blot analysis Protein was extracted from rat small intestine and IEC-6 cells using a total protein extraction kit (#K3011010, BioChain, Hayward, CA, USA), and the concentration determined using a bicinchoninic acid protein assay (#23225, Pierce, Rockford, IL, USA). …
- Automation of Dimethylation-after-Guanidination (2MEGA) Labeling Chemistry and Its Compatibility to Common Buffers and Surfactants for Mass Spectrometry-Based …
Lo, Tang, Chen, Li
Analytica Chimica Acta 0, , ,
… Rapigest™ was purchased from Waters (Milford, MA). Anionic acid labile surfactant (AALS) was obtained from Canadian Life Sciences (Peterborough, ON). Total protein extraction kit was purchased from BioChainInstitute (Hayward, CA). 2.2. Protein Sample Preparation. …
- Reduction of intestinal mucosal immune function in heat-stressed rats and bacterial translocation
Xiaoxi Liu, Huanrong Li, An Lu, Yougang Zhong, Xiaolin Hou, Ning Wang, Dan Jia, Junlan Zan, Hong Zhao, Jianqin Xu, Fenghua Liu
Int J Hyperthermia (2.9), 2012-11-16, 28, 756-65
… Thus, protein from rat jejunum was extracted by a total protein extraction kit (K3011010, BioChain, San Francisco, CA, USA). The concentration of extracted protein was quantified by a BCA protein assay kit (23225, Pierce, Thermo Scientific, Rockford, IL, USA). …
- Resveratrol regulates N-methyl-D-aspartate receptor expression and suppresses neuroinflammation in morphine-tolerant rats
Ru-Yin Tsai, Kuang-Yi Chou, Ching-Hui Shen, Chih-Cheng Chien, Wei-Yuan Tsai, Ya-Ni Huang, Pao-Luh Tao, Yaoh-Shiang Lin, Chih-Shung Wong
Analg. (3.3), 2012-10-24, 115, 944-52
… The dorsal portion of the lumbar spinal cords was fractionated into cytosolic, membrane, and nuclear fractions using a cytoplasmic, nuclear, and membrane compartment protein extraction kit, as recommended by the manufacturer (BioChainInstitute, Inc., Hayward, CA). …
- miR-548c-5p inhibits proliferation and migration and promotes apoptosis in CD90(+) HepG2 cells
Lin Fang, Hai-Bing Zhang, Hua Li, Yong Fu, Guang-Shun Yang
Radiol Oncol (2), 2012-09-18, 46, 233-41
… and caspase-8). Caspase-3, bcl-2 and bcl-xl protein expression Total protein was extracted at 48 h after the trans- fection with Total Protein Extraction Kit (BioChain, USA) according to manufacturer’s instructions. In brief, 100 …
- Negative regulation of human astrocytes by interferon (IFN) α in relation to growth inhibition and impaired glucose utilization
Ting Wang, Yasuhiro Takikawa, Kei Sawara, Yuichi Yoshida, Kazuyuki Suzuki
Res. (2.6), 2012-09-01, 37, 1898-905
… Western Blot Analysis Total protein was isolated from astrocytes by a total protein extraction kit purchased from BioChainInstitute, Inc. Protein from each sample (10 μg) was separated by 10 % SDS-PAGE and electrotransferred onto a polyvinylidene difluoride
- Carnosic acid (CA) prevents lipid accumulation in hepatocytes through the EGFR/MAPK pathway
Ting Wang, Yasuhiro Takikawa, Takahito Tabuchi, Takumi Satoh, Kunio Kosaka, Kazuyuki Suzuki
J. Gastroenterol. (3.6), 2012-07-19, 47, 805-13
… tractant protein 1 (MCP-1). Western blot analysis Total protein was isolated from liver tissue or HepG2 cells by a total protein extraction kit purchased from BioChain Institute, Inc. (Hayward, CA, USA). The nuclear fraction was …
- Overexpression of extracellular superoxide dismutase has a protective role against hyperoxia-induced brain injury in neonatal mice
Nahla Zaghloul, Mansoor Nasim, Hardik Patel, Champa Codipilly, Philippe Marambaud, Stephen Dewey, Wynne K Schiffer, Mohamed Ahmed
FEBS J. (3.1), 2012-03-21, 279, 871-81
… Brains were isolated and immediately frozen after exposure to hyperoxia or RA. Frozen tissues were crushed and homogenized, and protein extraction was carried out with a Total Protein Extraction Kit (BioChainInstitute, Hayward, CA, USA). …
- Ali Ö. Yildirim, Vandana Muyal, Gerrit John, Bernd Müller, Carola Seifart, Michael Kasper, and Heinz Fehrenbach
Palifermin Induces Alveolar Maintenance Programs in Emphysematous Mice
Am. J. Respir. Crit. Care Med., Apr 2010; 181: 705 – 717.
… …details.) Protein Isolation and Immunoblotting Protein was extracted from lung tissue using total protein extraction kit (Biochain Institute, Hayward, CA). Protein concentrations were measured using BCA protein assay kit (Pierce, Rockford, IL). After fragmentation… …
- Ali O Yildirim, Vandana Muyal, Gerrit John, Bernd Mueller, Carola Seifart, Michael Kasper, and Heinz Fehrenbach
Palifermin Induces Alveolar Maintenance Programs in Emphysematous Mice
J. Respir. Crit. Care Med., Dec 2009; 10.1164/rccm.200804-573OC.
… …extracted from lung tissue using total protein extraction kit (BiochainInstitute, Hayward, USA). Protein concentrations were measured using BCATM protein assay kit (Pierce, Rockford, USA). After fragmentation…extracted from lung tissue using total protein extraction kit (Biochain Institute, Hayward, USA) according to the manufacturer’s protocol… …
- Guomin Jiang, Yan Ke, Deming Sun, Hao Li, Mark Ihnen, Marcia M. Jumblatt, Gary Foulks, Yali Wang, Yang Bian, Henry J. Kaplan, and Hui Shao
A New Model of Experimental Autoimmune Keratoconjunctivitis Sicca (KCS) Induced in Lewis Rat by the Autoantigen Klk1b22
Ophthalmol. Vis. Sci., May 2009; 50: 2245 – 2254.
… …glands and salivary glands of B6 mice by homogenization and sonication following the method of a total protein extraction kit (BioChainInstitute, Inc., Hayward, CA). Briefly, the glands were isolated from PBS perfused mice, cut into small pieces, and incubated… …
- Wu, S. Chang, Y. Chen, Q. Xia, M. Forbes, and J. C. Tsai
Erythropoietin Receptor Plays a Role in the Cell Differentiation of Retina and Lens During Eye Development
Invest. Ophthalmol. Vis. Sci., Apr 2008; 49: 3078.
… …without retina). All collected tissues were stored at -70C for tissue protein preparation using a whole protein extract kit (Biochain Institute, Inc.). The total protein lysate (15microl) was subject to 12% SDS-PAGE followed by western blotting procedure with… …
- Emily I. Chen, Johannes Hewel, Joseph S. Krueger, Claire Tiraby, Martin R. Weber, Anastasia Kralli, Katja Becker, John R. Yates, III, and Brunhilde Felding-Habermann
Adaptation of Energy Metabolism in Breast Cancer Brain Metastases
Cancer Res., Feb 2007; 67: 1472 – 1486.
… …extracts were prepared with the TotalProteinExtraction kit (BioChain, Hayward, CA) and protein concentration was determined…extracts were prepared with the TotalProteinExtraction kit (BioChain) and protein concentrations determined by BCA assay (Pierce… …
- Pinthus JH, Bryskin I, Trachtenberg J, Lu JP, Singh G, Fridman E, Wilson BC.
Androgen Induces Adaptation to Oxidative Stress in Prostate Cancer: Implications for Treatment with Radiation Therapy.
2007 Jan; 9(1): 68-80.
… …Centrifuging at 12,000g for 15 minutes separated insoluble materials. Supernatants were dissolved in Laemmli sample buffer. Protein purification from tumor tissues was performed using a protein extraction kit (Biochain Institute, Inc., Greenland, NH)…. …
- Jia-Perng Chen, Hsin-Lin Lu, Szu-Liang Lai, Gabriele S. Campanella, Jui-Ming Sung, Mei-Yi Lu, Betty A. Wu-Hsieh, Yi-Ling Lin, Thomas E. Lane, Andrew D. Luster, and Fang Liao
Dengue Virus Induces Expression of CXC Chemokine Ligand 10/IFN–Inducible Protein 10, Which Competitively Inhibits Viral Binding to Cell Surface Heparan Sulfate
Immunol., Sep 2006; 177: 3185 – 3192.
… …the reference were approximately equal. ELISA Livers were weighed and homogenized on ice in protein extraction buffer (BioChainInstitute) using a tapered tissue grinder (Wheaton Science Products). The resultant homogenates were centrifuged at 15,000… …
- Chang, R. Yamada, A. Suzuki, Y. Kochi, T. Sawada, and K. Yamamoto
Citrullination of fibronectin in rheumatoid arthritis synovial tissue
Rheumatology, Nov 2005; 44: 1374 – 1382.
… …the Total Protein Extraction Kit (Biochain, USA) according to the manufacturers…prostate and thymus adenocarcinoma (BioChain). Fn in the total proteins was precipitated…the Total Protein Extraction Kit (Biochain, USA) according to the manufacturer’s… …
- Emily I. Chen, Laurence Florens, Fumiko T. Axelrod, Edward Monosov, Carlos F. Barbas III, John R. Yates III, Brunhilde Felding-Habermann, and Jeffrey W. Smith
Maspin alters the carcinoma proteome
FASEB J, Apr 2005; 10.1096/fj.04-2970fje.
… …using TotalProteinExtraction kit (BioChain, Hayward, CA), and protein concentration…homogenized in the lysis buffer from the Biochain total protein extraction kit (Biochain). Cellular 20S proteasome activity… …
- Chang, R. Yamada, A. Suzuki, T. Sawada, S. Yoshino, S. Tokuhiro, and K. Yamamoto
Localization of peptidylarginine deiminase 4 (PADI4) and citrullinated protein in synovial tissue of rheumatoid arthritis
Rheumatology, Jan 2005; 44: 40 – 50.
… we purified total protein of RA synovial tissues and leucocytes using total protein extraction kits (Biochain). …