• An electrocardiographic, molecular and biochemical approach to explore the cardioprotective effect of vasopressin and milrinone against phosphide toxicity in rats
  A Jafari, A Baghaei, R Solgi, M Baeeri… – Food and Chemical …, 2015 – Elsevier
  … Received 23 January 2015, Accepted 25 February 2015, Available online 18 March
  Highlights. • … (Tehran, Iran) were used in this study. The mitochondria isolation
  kit was purchased from BioChain Inc. (Newark, New Jersey, USA). …
• A quantitative assay for reductive metabolism of a pesticide in fish using electrochemistry coupled with tandem mass spectrometry
  U Bussy, YW Chung-Davidson, K Li… – Environmental Science & …, 2015 – ACS Publications
  …Tissue Extractions and Protein Concentration Determination Compartmental protein and
  mitochondria were extracted using the Mitochondria Isolation Kit and Compartmental Protein
  Extraction Kit (BioChain, Newark, CA) according to the manufacturer’s instructions. …
• A thermogenic secondary sexual character in male sea lamprey
  Yu-Wen Chung-Davidson, M Cody Priess, Chu-Yin Yeh, Cory O Brant, Nicholas S Johnson, Ke Li, Kaben G Nanlohy, Mara B Bryan, C Titus Brown, Jongeun Choi, Weiming Li
  Exp. Biol. (3), 2013-07-15, 216, 2702-12
  … Louis, MO, USA). Mitochondrial assays. Mitochondria were extracted from rope, gill and muscle tissues using a mitochondria isolation kit following the manufacturer’s instructions (BioChainInstitute, Newark, CA, USA). Briefly, 100 ..
• Mechanisms of muscular electrophysiological and mitochondrial dysfunction following exposure to malathion, an organophosphorus pesticide
  S Karami-Mohajeri, Mr Hadian, S Fouladdel, E Azizi, Mh Ghahramani, R Hosseini, M Abdollahi
  Hum Exp Toxicol (1.2), 2013-06-17, ,
  … Materials and methods. Materials. Pure malathion (technical grade ˜95%) was obtained from Shimi Keshavarz pesticide production Co. (Tehran, Iran). Mitochondria isolation kit was from BioChain (Newark, New Jersey, USA). …
• Hyperthyroidism causes cardiac dysfunction by mitochondrial impairment and energy depletion
  Sangeeta Maity, Dipak Kar, Kakali De, Vivek Chander, Arun Bandyopadhyay
  Endocrinol. (3.1), 2013-05-16, 217, 215-28
  … leupeptin, and aprotinin. Protein from mitochondrial fraction was isolated using Mitochondria Isolation Kit for Tissue and Cultured Cells (BioChainInstitute, Inc., Hayward, CA, USA) as per the manufacturer’s protocol. Equal amounts …
• Mitochondrial-dependent anticancer activity of δ-tocotrienol and its synthetic derivatives in HER-2/neu overexpressing breast adenocarcinoma cells
  Valentina Viola, Silvia Ciffolilli, Silvia Legnaioli, Marta Piroddi, Michele Betti, Francesco Mazzini, Elisa Pierpaoli, Mauro Provinciali, Francesco Galli
  Biofactors (2.8), 2013-01-30, ,
  … Mitochondrial were isolated using a kit from BioChainInstitute, Hayward, CA. 2.2. Cells and Treatments. Human and murine HER-2/neu proto-oncogene overexpressing breast cancer cells (SKBR3 and TUBO, respectively), were used to assess anti-cancer effects of VE forms. …
• Solution structure and siRNA-mediated knockdown analysis of the mitochondrial disease-related protein C12orf65
  Hiroyuki Kogure, Yusuke Hikawa, Mamoru Hagihara, Naoya Tochio, Seizo Koshiba, Yusuke Inoue, Peter Güntert, Takanori Kigawa, Shigeyuki Yokoyama, Nobukazu Nameki
  Proteins (2.8), 2012-11-04, 80, 2629-42
  … Cytochrome c oxidase assay. The cytochrome c oxidase assay was conducted using the Mitochondria Isolation Kit for Cultured Cells (PIERCE) and the cytochrome c oxidase activity assay kit (BioChain) according to the manufacturers’ instructions. Results. …
• Supply-side constraints are insufficient to explain the ontogenetic scaling of metabolic rate in the tobacco Hornworm, Manduca sexta
  Viviane Callier, H Frederik Nijhout
  PLoS ONE (4.4), 2012-10-02, 7, e45455
  … To determine the contribution of mitochondrial activity to the decrease in mass-specific metabolic rate of Manduca, we used a mitochondrial isolation and COX activity assay (BioChainkits # KC010100 and #KC310100) and followed the instructions in the kit (the technique is …
• Mitochondrial proteomic analysis reveals deficiencies in oxygen utilization in medullary thick ascending limb of Henle in the Dahl salt-sensitive rat
  Nadezhda N Zheleznova, Chun Yang, Robert P Ryan, Brian D Halligan, Mingyu Liang, Andrew S Greene, Allen W Cowley
  Genomics (3.4), 2012-09-01, 44, 829-42
  … The BioChainmitochondria isolation kit (cat. … In brief, mTALs that were bulk isolated as described above from both kidneys were resuspended into a mitochondria isolation buffer for extraction of mitochondrial proteins (mitochondria isolation kit, BioChain, KC010100). …
• Ischemia-reperfusion induces myocardial infarction through mitochondrial Ca²⁺ overload
  Kaori Shintani-Ishida, Makoto Inui, Ken-Ichi Yoshida
  Mol. Cell. Cardiol. (5.5), 2012-08-03, 53, 233-9
  … The mitochondria (approximately 0.2 mg) isolated using a Mitochondria Isolation Kit (BioChain, Hayward, CA) were preincubated at 30 °C for 10 min in medium containing 110 mM KCl, 20 mM MOPS, 10 mM Tris–HCl, 0.5 μM rotenone and 0.5 μM antimycin (pH 7.4). …
• Coenzyme Q10 reverses mitochondrial dysfunction in atorvastatin-treated mice and increases exercise endurance
  Ayako Muraki, Kazutoshi Miyashita, Masanori Mitsuishi, Masanori Tamaki, Kumiko Tanaka, Hiroshi Itoh
  Appl. Physiol. 0, 2012-08-02, 113, 479-86
  … Isolation of mitochondria and evaluation of mitochondrial activity. Mitochondria were isolated from the tissues or cultured cells using a commercially available kit (KC010100, BioChainInstitute, Hayward, CA), according to the manufacturer’s instructions. …
• Angiotensin II-induced mitochondrial Nox4 is a major endogenous source of oxidative stress in kidney tubular cells
  Su-Mi Kim, Yang-Gyun Kim, Kyung-Hwan Jeong, Sang-Ho Lee, Tae-Won Lee, Chun-Gyoo Ihm, Ju-Young Moon
  PLoS ONE (4.4), 2012-07-18, 7, e39739
  … Isolation of Mitochondria (Mt). NRK-52E cells were harvested from cultures and fractionated into cytosol (Cyt) and mitochondria using a mitochondrial isolation kit (BioChainInstitute, Inc., CA, USA) according to the manufacturer’s instructions. …
• Rotenone-induced death of RGC-5 cells is caspase independent, involves the JNK and p38 pathways and is attenuated by specific green tea flavonoids
  T A Kamalden, D Ji, N N Osborne
  Res. (2.6), 2012-05-09, 37, 1091-101
  … All other chemicals and reagents were purchased from Sigma-Aldrich (St Louis, MO, USA). Mitochondria Isolation Kit was purchased from BioChainInstitute Inc (CA) and used for subcellular fractionation of RGC-5 cell lysates. Lipid Peroxidation …
• Interaction of α-synuclein with vesicles that mimic mitochondrial membranes
  Imola G Zigoneanu, Yoo Jeong Yang, Alexander S Krois, Emdadul Haque, Gary J Pielak
  Biophys. Acta 0, 2012-03-06, 1818, 512-9
  … from Pierce. Their activity was tested by assessing the cytochrome c oxidase activity with the Mitochondria Activity Assay Kit from BioChainInstitute, Inc. (Hayward, CA). 2.3. Mitochondrial import of α-synuclein. Freshly isolated …
• A novel neuroprotective therapy for Parkinson’s disease using a viral noncoding RNA that protects mitochondrial complex I activity
  Wei-Li Kuan, Emma Poole, Michael Fletcher, Sharon Karniely, Pam Tyers, Mark Wills, Roger A Barker, John H Sinclair
  Exp. Med. (14.8), 2012-01-16, 209, 1-10
  … glucose, and 1 mM kynurenic acid). Mitochondrial preparations were obtained from brain sections using a proprietary mitochondria isolation kit (BioChainInstitute) as described by the manufacturer. For analysis of Complex I …
• Samik Bindu, Chinmay Pal, Sumanta Dey, Manish Goyal, Athar Alam, Mohd. Shameel Iqbal, Shubham Dutta, Souvik Sarkar, Rahul Kumar, Pallab Maity, and Uday Bandyopadhyay
  Translocation of Heme Oxygenase-1 to Mitochondria Is a Novel Cytoprotective Mechanism against Non-steroidal Anti-inflammatory Drug-induced Mitochondrial Oxidative Stress, Apoptosis, and Gastric Mucosal Injury
  Biol. Chem., Nov 2011; 286: 39387 – 39402.
  … …antioxidant response element (ARE) sequence were purchased from Sigma Genosys. The mitochondrial isolation kit was purchased from BiochainInstitute (Hayward, CA). TRIzol and Alexa Fluor 647 anti-rabbit antibodies were purchased from Invitrogen. Cytochrome c oxidase… …
• Iori Ueki, Heather B. Roman, Alessandro Valli, Krista Fieselmann, Jimmy Lam, Rachel Peters, Lawrence L. Hirschberger, and Martha H. Stipanuk
  Knockout of the murine cysteine dioxygenase gene results in severe impairment in ability to synthesize taurine and an increased catabolism of cysteine to hydrogen sulfide
  Am J Physiol Endocrinol Metab, Oct 2011; 301: E668 – E684.
  … …Cytochrome c oxidase activity in the isolated mitochondria was assessed using a commercial activity assay kit (no. KC310100; BioChainInstitute, Hayward, CA). Plasma IGF-I and blood acylcarnitine measurements. Plasma IGF-I was measured using the Luminex… …
• Hai-Chun Yang, Sebastien Deleuze, Yiqin Zuo, Sebastian A. Potthoff, Li-Jun Ma, and Agnes B. Fogo
  The PPAR  Agonist Pioglitazone Ameliorates Aging-Related Progressive Renal Injury
  Am. Soc. Nephrol., Nov 2009; 20: 2380 – 2388.
  … …activity in the soluble and membrane-bound mitochondria sample was assayed for mitochondria activity using a commercial kit (BiochainInstitute, Hayward, CA). Mitochondrial DNA Deletion Cortical tissue (100 mg) was homogenized, and mitochondrial DNA was extracted… …