Below is a list of some published scientific papers that used protein extraction kits:

 

  • Distinct Patterns of Wnt3a and Wnt5a Signaling Pathway in the Lung from Rats with Endotoxic Shock
    HP Hii, MH Liao, SJ Chen, CC Wu, CC Shih – PloS one, 2015… Received: March 18, 2015; Accepted: July 10, 2015; Published: July 28, 2015. Copyright: © 2015 Hii et al. … the expressions of Wnt canonical or noncanonical signaling molecules in the lung were obtained by a CNM compartmental protein extraction kit (BioChain Inc., San …
  • Valproic acid attenuates intercellular adhesion molecule-1 and E-selectin through a chemokine ligand 5 dependent mechanism and subarachnoid hemorrhage …
    CZ Chang, SC Wu, CL Lin, AL Kwan – Journal of Inflammation, 2015– biomedcentral.com
     Submission date 5 December 2014 Acceptance date 24 March 2015 Article URL
    http://dx.doi.org/10.1186/s12950-015-0074-3  (2015)12:27 Page 2.  San Diego, CA 92195–3308,
    CNM protein extraction kits were from Biochain (K3012010, Hayward, CA 94545, USA). 
  • A quantitative assay for reductive metabolism of a pesticide in fish using electrochemistry coupled with tandem mass spectrometry
    U Bussy, YW Chung-Davidson, K Li… – Environmental Science & …, 2015 – ACS Publications
    Tissue Extractions and Protein Concentration Determination Compartmental protein and
    mitochondria were extracted using the Mitochondria Isolation Kit and Compartmental Protein
    Extraction Kit (BioChain, Newark, CA) according to the manufacturer’s instructions. 
  • Inhibition of DOR prevents remifentanil induced postoperative hyperalgesia through regulating the trafficking and function of spinal NMDA receptors in vivo and in vitro
    C Wang, Y Li, H Wang, K Xie, R Shu, L Zhang, N Hu… – Brain research …, 2015 – Elsevier
    ). Then lysate was then centrifuged and supernatant was removed as the total
    protein. To extract membrane protein, a membrane compartment protein extraction
    kit (BiochainInstitute, Inc., Hayward, CA) was used.
  • Cerium oxide nanoparticles induce cytotoxicity in human hepatoma SMMC-7721 cells via oxidative stress and the activation of MAPK signaling pathways
    Guilin Cheng, Wei Guo, Lu Han, Erlei Chen, Lingfang Kong, Lili Wang, Wenchao Ai, Naining Song, Haishan Li, Huiming Chen
    Toxicol In Vitro (2.5), 2013-04-25, 27, 1082-8
    … 2.6. Western blot analyses. Protein was extracted from SMMC-7721 cells using a total protein extraction kit (BioChain, Hayward, CA, USA) after each treatment; the concentration was determined using a bicinchoninic acid (BCA) protein assay (Pierce, Rockford, IL, USA). …
  • Screening citrullinated proteins in synovial tissues of rheumatoid arthritis using 2-dimensional western blotting
    Xiaotian Chang, Yan Zhao, Yuejian Wang, Yu Chen, Xinfeng Yan
    Rheumatol. (3.6), 2013-03-04, 40, 219-27
    … the sample loading. Immunoprecipitation. Total protein was purified from the synovial tissues of patients with RA (n = 5) using the Total Protein Extraction kit (BioChain) according to the manufacturer’s instructions. A1AT, keratin …
  • Involvement of oxidative stress and mitogen-activated protein kinase signaling pathways in heat stress-induced injury in the rat small intestine
    Jin Yu, Fenghua Liu, Peng Yin, Hong Zhao, Weili Luan, Xiaolin Hou, Yougang Zhong, Dan Jia, Junlan Zan, Wuren Ma, Banchao Shu, Jianqin Xu
    Stress (2.6), 2013-01-11, 16, 99-113
    … Western-blot analysis Protein was extracted from rat small intestine and IEC-6 cells using a total protein extraction kit (#K3011010, BioChain, Hayward, CA, USA), and the concentration determined using a bicinchoninic acid protein assay (#23225, Pierce, Rockford, IL, USA). …
  • Ubiquitin C-terminal hydrolase-L5 is required for high glucose-induced transforming growth factor-β receptor I expression and hypertrophy in mesangial cells
    Yu-Min Ko, Chun-Ying Chang, Shean-Jaw Chiou, Fu-Jie Hsu, Jau-Shyang Huang, Yu-Lin Yang, Jinn-Yuh Guh, Lea-Yea Chuang
    Biochem. Biophys. (3), 2013-07-15, 535, 177-86
    … Briefly, the cells were rinsed twice with ice-cold phosphate-buffered saline, trypsinized, pelleted, and fractionated into cytosolic and nuclear fractions with the CNM compartment protein extraction kit (BioChainInstitute, Inc., Hayward, CA) as recommended by the manufacturer. …
  • Tumor protein D54 is a negative regulator of extracellular matrix-dependent migration and attachment in oral squamous cell carcinoma-derived cell lines
    Yoshiki Mukudai, Seiji Kondo, Atsushi Fujita, Yasuto Yoshihama, Tatsuo Shirota, Satoru Shintani
    Cell Oncol (Dordr) 0, 2013-06-17, 36, 233-45
    … Total cellular proteins were prepared as described before [32] and cell-fraction proteins were extracted using a commercial kit (CNMS Compartmental Protein Extraction Kit, BioChainInstitute, Hayward, CA) according to the manufacturer’s pro- tocol. …
  • MR Signal Amplification for Imaging of the Mutant EGF Receptor in Orthotopic Human Glioma Model
    Mohammed Salman Shazeeb, Suresh Gupta, Alexei Bogdanov
    Mol Imaging Biol (3.1), 2013-06-04, ,
    … Cell membrane proteins were extracted using a CNM compartmental protein extraction kit (BioChainInstitute Inc., Hayward, CA) and normalized per protein concentration using a Micro BCA Protein Assay kit (Thermo Scientific). …
  • Nucleophosmin, a critical Bax cofactor in ischemia-induced cell death
    Zhiyong Wang, Jonathan M Gall, Ramon Bonegio, Andrea Havasi, Katarina Illanes, John H Schwartz, Steven C Borkan
    Cell. Biol. (6.2), 2013-05-24, 33, 1916-24
    … digitonin (8 μg/ml). Cytosolic proteins were extracted from renal cortical samples using a commercially available tissue protein extraction kit (BioChainInstitute, Newark, CA) by following the manufacturer’s instructions. Briefly, 50 to …
  • Functional proteomics reveals the protective effects of saffron ethanolic extract on hepatic ischemia-reperfusion injury
    Tai-Long Pan, Tung-Ho Wu, Pei-Wen Wang, Yann-Lii Leu, Nardnisa Sintupisut, Chun-Hsun Huang, Fang-Rong Chang, Yang-Chang Wu
    Proteomics (4.8), 2013-05-21, ,
    … According to the manufacturer’s instructions, the liver tissue was homogenized in ice-cold buffer C (CNM compartment protein extraction kit, BioChainInstitute). Samples from cytosol and membrane fractions were denatured and then subjected to SDS-PAGE. The translocation of …
  • Baicalin ameliorates neuropathic pain by suppressing HDAC1 expression in the spinal cord of spinal nerve ligation rats
    Chen-Hwan Cherng, Kwong-Chiu Lee, Chih-Cheng Chien, Kuang-Yi Chou, Yu-Che Cheng, Shih-Tai Hsin, Sing-Ong Lee, Ching-Hui Shen, Ru-Yin Tsai, Chih-Shung Wong
    Formos. Med. Assoc. (1.1), 2013-05-15, ,
    … The dorsal part of the ipsilateral spinal cord was fractionated into cytosolic, membrane, and nuclear fractions using a cytoplasmic, nuclear, and membrane (CNM) compartment protein extraction kit as recommended by the manufacturer (BioChainInstitute Inc., Hayward, CA, USA …
  • Proteasome dysfunction mediates obesity-induced endoplasmic reticulum stress and insulin resistance in the liver
    Toshiki Otoda, Toshinari Takamura, Hirofumi Misu, Tsuguhito Ota, Shigeo Murata, Hiroto Hayashi, Hiroaki Takayama, Akihiro Kikuchi, Takehiro Kanamori, Kosuke R Shima, Fei Lan, Takashi Takeda, Seiichiro Kurita, Kazuhide Ishikura, Yuki Kita, Kaito Iwayama, Ken-ichiro Kato, Masafumi Uno, Yumie Takeshita, Miyuki Yamamoto, Kunpei Tokuyama, Shoichi Iseki, Keiji Tanaka, Shuichi Kaneko
    Diabetes (8.9), 2013-03-22, 62, 811-24
    … Nuclear matrix and cell membrane fractions were extracted from whole cell lysates by using the CNMCS compartmental protein extraction kit (BioChain Institute) according to the manufacturer’s protocol. Electron microscopy. …
  • Expression of Notch receptor and its target gene Hes-1 in bone marrow CD34+ cells from patients with psoriasis
    Guohua Yin, Ruixia Hou, Junqin Li, Jing Zhang, Xinhua Li, Kaiming Zhang
    Dermatology (Basel) (2.7), 2012-12-04, 225, 147-53
    … goto top of outline Extraction of Total RNA and Protein from CD34+ Cells. The total RNA and protein of the same specimen were extracted simultaneously using the Dr. P kit (BioChain; Hayward, Hayward, Calif., USA) following the manufacturer’s recommendations. …
  • Eight new mutations and the expanding phenotype variability in muscular dystrophy caused by ANO5
    S Penttilä, J Palmio, T Suominen, O Raheem, A Evilä, N Muelas Gomez, G Tasca, L B Waddell, N F Clarke, A Barboi, P Hackman, B Udd
    Neurology (8), 2012-03-20, 78, 897-903
    … RNA was extracted from muscle tissue with a Dr. P Kit (BioChain, Hayward, CA) for Finnish patients or by a modified TRIzol extraction protocol (TRI Reagent; Molecular Research Center, Cincinnati, OH) for Australian patients. …
  • Genotoxic agents promote the nuclear accumulation of annexin A2: role of annexin A2 in mitigating DNA damage
    Patricia A Madureira, Richard Hill, Patrick W K Lee, David M Waisman
    PLoS ONE (4.4), 2012-12-11, 7, e50591
    … Nuclear/Cytoplasmic/Membrane/CytoskeletonFractionation. For multi-compartmental fractionation of cells we used the CNMS compartment protein extraction kit (BioChainInstitute) according to the manufacturer’s instructions. …
  • Phthalates stimulate the epithelial to mesenchymal transition through an HDAC6-dependent mechanism in human breast epithelial stem cells
    Tsung-Hua Hsieh, Cheng-Fang Tsai, Chia-Yi Hsu, Po-Lin Kuo, Jau-Nan Lee, Chee-Yin Chai, Ming-Feng Hou, Chia-Cheng Chang, Cheng-Yu Long, Ying-Chin Ko, Eing-Mei Tsai
    Sci. 0, 2012-08-16, 128, 365-76
    … Subcellular fractionation. To assess protein translocation, the nuclearand cytoplasmic fractions of R2d cells were isolated using the Compart mental Protein Extraction kit CNMCS (BioChain, Hayward, CA) according to the manufacturer’s protocol. …
  • Galectin-1 promotes lung cancer progression and chemoresistance by upregulating p38 MAPK, ERK, and cyclooxygenase-2
    Ling-Yen Chung, Shye-Jye Tang, Guang-Huan Sun, Teh-Ying Chou, Tien-Shun Yeh, Sung-Liang Yu, Kuang-Hui Sun
    Cancer Res. (7.3), 2012-08-01, 18, 4037-47
    … secondary antibodies. The antibodies used in this study are shown in Supplementary Table S5. Cytoplasm and nucleus of cells were separated by using the CNMCS Compartmental Protein Extraction Kit (BioChain). Whole-cell …
  • Increased Cdk5/p35 activity in the dentate gyrus mediates depressive-like behaviour in rats
    Wei-Li Zhu, Hai-Shui Shi, Shen-Jun Wang, Chun-Mei Xu, Wen-Gao Jiang, Xi Wang, Ping Wu, Qian-Qian Li, Zeng-Bo Ding, Lin Lu
    J. Neuropsychopharmacol. (4.7), 2012-07-11, 15, 795-809
    … Beyotime Biotechnology, China). The cytoplasmic and membrane fractions were sep- arated using the CNM (cytoplasmic, nuclear, mem- brane) Compartment Protein Extraction kit (K3012010, BioChainInstitute, USA). The protein …
  • Allosteric modulation of beta1 integrin function induces lung tissue repair
    Rehab Aljamal-Naylor, Linda Wilson, Susan McIntyre, Fiona Rossi, Beth Harrison, Mark Marsden, David J Harrison
    Adv Pharmacol Sci 0, 2012-04-16, 2012, 768720
    … At the end of the stretch, the media was aspirated, and protein extracted from the cell layer was fractionated using the compartmental protein extraction kit (CNMCS, BioChain). Protein content was measured using the BCA methods. …
  • Amitriptyline attenuates astrocyte activation and morphine tolerance in rats: role of the PSD-95/NR1/nNOS/PKCγ signaling pathway
    Ya-Ni Huang, Ru-Yin Tsai, Shinn-Long Lin, Chih-Cheng Chien, Chen-Hwan Cherng, Ching-Tang Wu, Chun-Chang Yeh, Chih-Shung Wong
    Brain Res. (3.4), 2012-04-15, 229, 401-11
    … fraction preparation, cytosolic and membrane fractions of the dorsal portion of the lumbar spinal cord enlargement were prepared using a Cytoplasmic, Nuclear, and Membrane compartment protein extraction kit as recommended by the manufacturer (BioChainInstitute, Inc …
  • Ultra-low-dose naloxone enhances the antinociceptive effect of morphine in PTX-treated rats: regulation on global histone methylation
    Ru-Yin Tsai, Ching-Hui Shen, Yu-Pin Feng, Chih-Cheng Chien, Sing-Ong Lee, Wei-Yuan Tsai, Yaoh-Shiang Lin, Chih-Shung Wong
    Acta Anaesthesiol Taiwan 0, 2012-09-02, 50, 106-11
    … The dorsal part of the spinal cord was fractionated into cytosolic, nuclear, and membrane fractions using a cytoplasmic, nuclear, and membrane compartment protein extraction as recommended by the manufacturer (BioChainInstitute, Inc., Hayward, CA, USA) and stored at …
  • Phthalates induce proliferation and invasiveness of estrogen receptor-negative breast cancer through the AhR/HDAC6/c-Myc signaling pathway
    Tsung-Hua Hsieh, Cheng-Fang Tsai, Chia-Yi Hsu, Po-Lin Kuo, Jau-Nan Lee, Chee-Yin Chai, Shao-Chun Wang, Eing-Mei Tsai
    FASEB J. (6.5), 2012-02-01, 26, 778-87
    … Cell fractionation. The nuclear, cytoplasmic, and membrane fractions were isolated using the CNMCS compartmental protein extraction kit (BioChain, Hayward, CA, USA) according to the manufacturer’s protocol. Chromatin immunoprecipitation (ChIP) assay. …
  • Explore the Molecular Mechanism of Apoptosis Induced by Tanshinone IIA on Activated Rat Hepatic Stellate Cells
    Tai-Long Pan, Pei-Wen Wang
    Evid Based Complement Alternat Med (3), 2012-01-24, 2012, 734987
    … previously [28]. Briefly, the 1 × 10 8 HSC-T6 cells with or without Tan IIA were suspended in ice-cold buffer C (CNM compartment protein extraction kit, BioChainInstitute) according to the manufacturer’s instructions. For cytoplasmic …
  • Anh-Nguyet T. Nguyen, Kyle Jansson, Gladis Sánchez, Madhulika Sharma, Gail A. Reif, Darren P. Wallace, and Gustavo Blanco
  • Automation of Dimethylation-after-Guanidination (2MEGA) Labeling Chemistry and Its Compatibility to Common Buffers and Surfactants for Mass Spectrometry-Based …
    Lo, Tang, Chen, Li
    Analytica Chimica Acta 0, , ,
    … Rapigest™ was purchased from Waters (Milford, MA). Anionic acid labile surfactant (AALS) was obtained from Canadian Life Sciences (Peterborough, ON). Total protein extraction kit was purchased from BioChainInstitute (Hayward, CA). 2.2. Protein Sample Preparation. …
  • Reduction of intestinal mucosal immune function in heat-stressed rats and bacterial translocation
    Xiaoxi Liu, Huanrong Li, An Lu, Yougang Zhong, Xiaolin Hou, Ning Wang, Dan Jia, Junlan Zan, Hong Zhao, Jianqin Xu, Fenghua Liu
    Int J Hyperthermia (2.9), 2012-11-16, 28, 756-65
    … Thus, protein from rat jejunum was extracted by a total protein extraction kit (K3011010, BioChain, San Francisco, CA, USA). The concentration of extracted protein was quantified by a BCA protein assay kit (23225, Pierce, Thermo Scientific, Rockford, IL, USA). …
  • Resveratrol regulates N-methyl-D-aspartate receptor expression and suppresses neuroinflammation in morphine-tolerant rats
    Ru-Yin Tsai, Kuang-Yi Chou, Ching-Hui Shen, Chih-Cheng Chien, Wei-Yuan Tsai, Ya-Ni Huang, Pao-Luh Tao, Yaoh-Shiang Lin, Chih-Shung Wong
    Analg. (3.3), 2012-10-24, 115, 944-52
    … The dorsal portion of the lumbar spinal cords was fractionated into cytosolic, membrane, and nuclear fractions using a cytoplasmic, nuclear, and membrane compartment protein extraction kit, as recommended by the manufacturer (BioChainInstitute, Inc., Hayward, CA). …
  • miR-548c-5p inhibits proliferation and migration and promotes apoptosis in CD90(+) HepG2 cells
    Lin Fang, Hai-Bing Zhang, Hua Li, Yong Fu, Guang-Shun Yang
    Radiol Oncol (2), 2012-09-18, 46, 233-41
    … and caspase-8). Caspase-3, bcl-2 and bcl-xl protein expression Total protein was extracted at 48 h after the trans- fection with Total Protein Extraction Kit (BioChain, USA) according to manufacturer’s instructions. In brief, 100 …
  • Negative regulation of human astrocytes by interferon (IFN) α in relation to growth inhibition and impaired glucose utilization
    Ting Wang, Yasuhiro Takikawa, Kei Sawara, Yuichi Yoshida, Kazuyuki Suzuki
    Res. (2.6), 2012-09-01, 37, 1898-905
    … Western Blot Analysis Total protein was isolated from astrocytes by a total protein extraction kit purchased from BioChainInstitute, Inc. Protein from each sample (10 μg) was separated by 10 % SDS-PAGE and electrotransferred onto a polyvinylidene difluoride
  • Carnosic acid (CA) prevents lipid accumulation in hepatocytes through the EGFR/MAPK pathway
    Ting Wang, Yasuhiro Takikawa, Takahito Tabuchi, Takumi Satoh, Kunio Kosaka, Kazuyuki Suzuki
    J. Gastroenterol. (3.6), 2012-07-19, 47, 805-13
    … tractant protein 1 (MCP-1). Western blot analysis Total protein was isolated from liver tissue or HepG2 cells by a total protein extraction kit purchased from BioChain Institute, Inc. (Hayward, CA, USA). The nuclear fraction was …
  • Overexpression of extracellular superoxide dismutase has a protective role against hyperoxia-induced brain injury in neonatal mice
    Nahla Zaghloul, Mansoor Nasim, Hardik Patel, Champa Codipilly, Philippe Marambaud, Stephen Dewey, Wynne K Schiffer, Mohamed Ahmed
    FEBS J. (3.1), 2012-03-21, 279, 871-81
    … Brains were isolated and immediately frozen after exposure to hyperoxia or RA. Frozen tissues were crushed and homogenized, and protein extraction was carried out with a Total Protein Extraction Kit (BioChainInstitute, Hayward, CA, USA). …
    Ouabain activates the Na-K-ATPase signalosome to induce autosomal dominant polycystic kidney disease cell proliferation
    Am J Physiol Renal Physiol, Oct 2011; 301: F897 – F906.
    … …fraction preparations. Nuclear extracts were prepared for determination of p21 and p27 using the CNM Compartment extraction kit (BiochainInstitute, Hayward, CA), following the maufacturer’s instructions. Briefly, ADPKD cells were treated in the absence or presence… …
  • Sheng-Tang Wu, Guang-Huan Sun, Chih-Ying Hsu, Chang-Shuo Huang, Yu-Hsin Wu, Hsiao-Hsien Wang, and Kuang-Hui Sun
    Tumor necrosis factor-αinduces epithelial–mesenchymal transition of renal cell carcinoma cells via a nuclear factor kappa B-independent mechanism
    Exp Biol Med, Sep 2011; 236: 1022 – 1029.
    … …cells were collected. The cytoplasm and nuclei of cells were separated by using the CNMCS compartmental protein extraction kit (BioChain, Hayward, CA, USA). The protein concentrations of the cell lysates were measured using the Bio-Rad protein assay kit. The proteins… …
  • Ching-Hui Shen, Ru-Yin Tsai, Yueh-Hwa Tai, Shinn-Long Lin, Chih-Cheng Chien, and Chih-Shung Wong
    Intrathecal Etanercept Partially Restores Morphine’s Antinociception in Morphine-Tolerant Rats via Attenuation of the Glutamatergic Transmission
    Analg., Jul 2011; 113: 184 – 190.
    … …fractions with the cytoplasmic, nuclear, and membrane compartment protein extraction kit as recommended by the manufacturer (BioChainInstitute, Inc., Hayward, CA). The membrane fraction was checked by Western blotting with anti-EGFR (epidermal growth factor… …
  • Peter Gruber, Florian Rechfeld, Johannes Kirchmair, Nina Hauser, Markus Boehler, Dorota Garczarczyk, Thierry Langer, and Johann Hofmann
    Barbituric acid derivative BAS 02104951 inhibits PKC , PKC , PKC /RACK2 interaction, Elk-1 phosphorylation in HeLa and PKC  and   translocation in PC3 cells following TPA-induction
    Biochem., Mar 2011; 149: 331 – 336.
    … …and activated with 100 nM TPA for 5 min. Cells were fractionated using the CNMCS/CNM compartmental Protein Extraction Kit (Biochain, Hayward, USA) according to the manufacturers protocol. 10 microg of proteins of the membrane fraction were used for SDS-PAGE…
  • Sandrine Vitry, Julie Bruyère, Michaël Hocquemiller, Stéphanie Bigou, Jérôme Ausseil, Marie-Anne Colle, Marie-Christine Prévost, and Jean Michel Heard
    Storage Vesicles in Neurons Are Related to Golgi Complex Alterations in Mucopolysaccharidosis IIIB
    J. Pathol., Dec 2010; 177: 2984 – 2999.
    …. …NaCl, 50 mmol/L Tris pH7.8, protease inhibitors). Membrane proteins were extracted with a CNMCS Compartmental extraction kit (Biochain, Hayward, US) according to manufacturer recommendations. Endo-H (Biolabs, Beverly, US) and peptide N-glycosidase F (PNGase… …
  • Chi-Wen Lo, Min-Wei Chen, Michael Hsiao, Shiuan Wang, Chi-An Chen, Sheng-Mou Hsiao, Jeng-Shou Chang, Tsung-Ching Lai, Stefan Rose-John, Min-Liang Kuo, and Lin-Hung Wei
    IL-6 Trans-Signaling in Formation and Progression of Malignant Ascites in Ovarian Cancer
    Cancer Res., Jan 2011; 71: 424 – 434.
    … …Tyr 685; ECM Biosciences). Cytoplasmic VE-cadherin protein was prepared by the CNMCS compartmental protein extraction kit (BioChain). Cytotoxicity assay Cell survival was determined using the MTT assay (Sigma). Assay of chemotactic endothelial cell… …link
  • Weijing Cai, Massimo Torreggiani, Li Zhu, Xue Chen, John Cijiang He, Gary E. Striker, and Helen Vlassara
    AGER1 regulates endothelial cell NADPH oxidase-dependent oxidant stress via PKC- : implications for vascular disease
    Am J Physiol Cell Physiol, Mar 2010; 298: C624 – C634.
    … …membranes and nuclear proteins were prepared from ECV304, HAEC cells, and mouse aortic segments using a kit purchased from BioChainInstitute (Hayward, CA). Cells were centrifuged, and the pellet was washed with PBS 3, and then ice-cold buffer C (HEPES pH…
  • Ali Ö. Yildirim, Vandana Muyal, Gerrit John, Bernd Müller, Carola Seifart, Michael Kasper, and Heinz Fehrenbach
    Palifermin Induces Alveolar Maintenance Programs in Emphysematous Mice
    Am. J. Respir. Crit. Care Med., Apr 2010; 181: 705 – 717.
    … …details.) Protein Isolation and Immunoblotting Protein was extracted from lung tissue using total protein extraction kit (Biochain Institute, Hayward, CA). Protein concentrations were measured using BCA protein assay kit (Pierce, Rockford, IL). After fragmentation… …
  • Ali O Yildirim, Vandana Muyal, Gerrit John, Bernd Mueller, Carola Seifart, Michael Kasper, and Heinz Fehrenbach
    Palifermin Induces Alveolar Maintenance Programs in Emphysematous Mice
    J. Respir. Crit. Care Med., Dec 2009; 10.1164/rccm.200804-573OC.
    … …extracted from lung tissue using total protein extraction kit (BiochainInstitute, Hayward, USA). Protein concentrations were measured using BCATM protein assay kit (Pierce, Rockford, USA). After fragmentation…extracted from lung tissue using total protein extraction kit (Biochain Institute, Hayward, USA) according to the manufacturer’s protocol… …
  • Guomin Jiang, Yan Ke, Deming Sun, Hao Li, Mark Ihnen, Marcia M. Jumblatt, Gary Foulks, Yali Wang, Yang Bian, Henry J. Kaplan, and Hui Shao
    A New Model of Experimental Autoimmune Keratoconjunctivitis Sicca (KCS) Induced in Lewis Rat by the Autoantigen Klk1b22
    Ophthalmol. Vis. Sci., May 2009; 50: 2245 – 2254.
    … …glands and salivary glands of B6 mice by homogenization and sonication following the method of a total protein extraction kit (BioChainInstitute, Inc., Hayward, CA). Briefly, the glands were isolated from PBS perfused mice, cut into small pieces, and incubated… …
  • Wu, S. Chang, Y. Chen, Q. Xia, M. Forbes, and J. C. Tsai
    Erythropoietin Receptor Plays a Role in the Cell Differentiation of Retina and Lens During Eye Development
    Invest. Ophthalmol. Vis. Sci., Apr 2008; 49: 3078.
    … …without retina). All collected tissues were stored at -70C for tissue protein preparation using a whole protein extract kit (Biochain Institute, Inc.). The total protein lysate (15microl) was subject to 12% SDS-PAGE followed by western blotting procedure with… …
  • Jennifer E. Bruin, Hertzel C. Gerstein, Katherine M. Morrison, and Alison C. Holloway
    Increased Pancreatic Beta-Cell Apoptosis following Fetal and Neonatal Exposure to Nicotine Is Mediated via the Mitochondria
    Sci., Jun 2008; 103: 362 – 370.
    … …Science, Laval, PQ). To separate mitochondrial and cytosolic fractions, the Compartmental Protein Extraction Kit (K3013010; Biochain Institute, Inc., Hayward, CA) was used according to manufacturer’s instructions. For Western blots of whole pancreas homogenates… …
  • Yingqiu Xie, Kexin Xu, Douglas E. Linn, Xi Yang, Zhiyong Guo, Hermela Shimelis, Takeo Nakanishi, Douglas D. Ross, Hegang Chen, Ladan Fazli, Martin E. Gleave, and Yun Qiu
    The 44-kDa Pim-1 Kinase Phosphorylates BCRP/ABCG2 and Thereby Promotes Its Multimerization and Drug-resistant Activity in Human Prostate Cancer Cells
    Biol. Chem., Feb 2008; 283: 3349 – 3356.
    … …for three times at 4 C with the lysis buffer. Cell fractionation was carried out using compartmental protein extraction kit (BioChain, Inc.). Immunoblotting was performed as described previously (24). Briefly, blots were incubated with primary antibodies, 1… …
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  • Emily I. Chen, Johannes Hewel, Joseph S. Krueger, Claire Tiraby, Martin R. Weber, Anastasia Kralli, Katja Becker, John R. Yates, III, and Brunhilde Felding-Habermann
    Adaptation of Energy Metabolism in Breast Cancer Brain Metastases
    Cancer Res., Feb 2007; 67: 1472 – 1486.
    … …extracts were prepared with the TotalProteinExtraction kit (BioChain, Hayward, CA) and protein concentration was determined…extracts were prepared with the TotalProteinExtraction kit (BioChain) and protein concentrations determined by BCA assay (Pierce… …
    link
  • Pinthus JH, Bryskin I, Trachtenberg J, Lu JP, Singh G, Fridman E, Wilson BC.
    Androgen Induces Adaptation to Oxidative Stress in Prostate Cancer: Implications for Treatment with Radiation Therapy.
    2007 Jan; 9(1): 68-80.
    … …Centrifuging at 12,000g for 15 minutes separated insoluble materials. Supernatants were dissolved in Laemmli sample buffer. Protein purification from tumor tissues was performed using a protein extraction kit (Biochain Institute, Inc., Greenland, NH)…. …
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  • Chun-Chun Li, Tsai-Chen Chiang, Tsung-Sheng Wu, Gustavo Pacheco-Rodriguez, Joel Moss, and Fang-Jen S. Lee
    ARL4D Recruits Cytohesin-2/ARNO to Modulate Actin Remodeling
    Mol. Biol. Cell, Sep 2007; 10.1091/mbc.E07-02-0149.
    … …DSP and then the cytosol and membrane fractions of COS-7 cells were prepared using a CNM compartment protein extraction kit (BioChain Institute Inc., Hayward, CA) according to the manufacturer’s instructions. Pull-down Assay for ARF6-GTP The activation levels… …
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  • Kasie K. Cole-Edwards, Alberto E. Musto, and Nicolas G. Bazan
    c-Jun N-Terminal Kinase Activation Responses Induced by Hippocampal Kindling Are Mediated by Reactive Astrocytes
    Neurosci., Aug 2006; 26: 8295 – 8304.
    … …Alternatively, to separate brain tissue into cytosolic, membrane, and nuclear protein fractions, a compartmental extraction kit from BioChain(Hayward, CA) was used. Tissue was homogenized in a Dounce homogenizer with five strokes of the loose-fitting pestle and manipulated… …
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  • Brian J. Siroky, William B. Ferguson, Amanda L. Fuson, Yi Xie, Attila Fintha, Peter Komlosi, Bradley K. Yoder, Erik M. Schwiebert, Lisa M. Guay-Woodford, and P. Darwin Bell
    Loss of primary cilia results in deregulated and unabated apical calcium entry in ARPKD collecting duct cells
    Am J Physiol Renal Physiol, Jun 2006; 290: F1320 – F1328.
    … …PC2 antibody. Cytosolic and membrane protein fractions were isolated using the CNM Compartment Protein Extraction Kit (BioChain). Proteins were separated using polyacrylamide electrophoresis and transferred onto PVDF membranes. Proteins labeled with… …
  • Jia-Perng Chen, Hsin-Lin Lu, Szu-Liang Lai, Gabriele S. Campanella, Jui-Ming Sung, Mei-Yi Lu, Betty A. Wu-Hsieh, Yi-Ling Lin, Thomas E. Lane, Andrew D. Luster, and Fang Liao
    Dengue Virus Induces Expression of CXC Chemokine Ligand 10/IFN–Inducible Protein 10, Which Competitively Inhibits Viral Binding to Cell Surface Heparan Sulfate
    Immunol., Sep 2006; 177: 3185 – 3192.
    … …the reference were approximately equal. ELISA Livers were weighed and homogenized on ice in protein extraction buffer (BioChainInstitute) using a tapered tissue grinder (Wheaton Science Products). The resultant homogenates were centrifuged at 15,000… …
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  • Chang, R. Yamada, A. Suzuki, Y. Kochi, T. Sawada, and K. Yamamoto
    Citrullination of fibronectin in rheumatoid arthritis synovial tissue
    Rheumatology, Nov 2005; 44: 1374 – 1382.
    … …the Total Protein Extraction Kit (Biochain, USA) according to the manufacturers…prostate and thymus adenocarcinoma (BioChain). Fn in the total proteins was precipitated…the Total Protein Extraction Kit (Biochain, USA) according to the manufacturer’s… …
  • Emily I. Chen, Laurence Florens, Fumiko T. Axelrod, Edward Monosov, Carlos F. Barbas III, John R. Yates III, Brunhilde Felding-Habermann, and Jeffrey W. Smith
    Maspin alters the carcinoma proteome
    FASEB J, Apr 2005; 10.1096/fj.04-2970fje.
    … …using TotalProteinExtraction kit (BioChain, Hayward, CA), and protein concentration…homogenized in the lysis buffer from the Biochain total protein extraction kit (Biochain). Cellular 20S proteasome activity… …
  • Chang, R. Yamada, A. Suzuki, T. Sawada, S. Yoshino, S. Tokuhiro, and K. Yamamoto
    Localization of peptidylarginine deiminase 4 (PADI4) and citrullinated protein in synovial tissue of rheumatoid arthritis
    Rheumatology, Jan 2005; 44: 40 – 50.
    … we purified total protein of RA synovial tissues and leucocytes using total protein extraction kits (Biochain). …
  • Christian Korn, Sebastian R. Scholz, Oleg Gimadutdinow, Rudi Lurz, Alfred Pingoud, and Gregor Meiss
    Interaction of DNA Fragmentation Factor (DFF) with DNA Reveals an Unprecedented Mechanism for Nuclease Inhibition and Suggests That DFF Can Be Activated in a DNA-bound State
    Chem., Feb 2005; 280: 6005 – 6015.
    … Endogenous DFF ?Non-detergent cell lysis was performed using the CNM compartmental protein extraction kit (BioChainInstitute, Inc.) according to the supplier’s recommendations. …
  • Neelam Sharma-Walia, Pramod P. Naranatt, Harinivas H. Krishnan, Ling Zeng, and Bala Chandran
    Kaposi’s Sarcoma-Associated Herpesvirus/Human Herpesvirus 8 Envelope Glycoprotein gB Induces the Integrin-Dependent Focal Adhesion Kinase-Src-Phosphatidylinositol 3-Kinase-Rho GTPase Signal Pathways and Cytoskeletal Rearrangements
    Virol., Apr 2004; 78: 4207 – 4223.
    … The CNM compartment protein extraction kit was obtained from the BiochainInstitute, Inc., Hayward, Calif. …
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