Involvement of oxidative stress and mitogen-activated protein kinase signaling pathways in heat stress-induced injury in the rat small intestine
Jin Yu, Fenghua Liu, Peng Yin, Hong Zhao, Weili Luan, Xiaolin Hou, Yougang Zhong, Dan Jia, Junlan Zan, Wuren Ma, Banchao Shu, Jianqin Xu
Stress (2.6), 2013-01-11, 16, 99-113
… Western-blot analysis Protein was extracted from rat small intestine and IEC-6 cells using a total protein extraction kit (#K3011010, BioChain, Hayward, CA, USA), and the concentration determined using a bicinchoninic acid protein assay (#23225, Pierce, Rockford, IL, USA). …
MR Signal Amplification for Imaging of the Mutant EGF Receptor in Orthotopic Human Glioma Model
Mohammed Salman Shazeeb, Suresh Gupta, Alexei Bogdanov
Mol Imaging Biol (3.1), 2013-06-04, ,
… Cell membrane proteins were extracted using a CNM compartmental protein extraction kit (BioChainInstitute Inc., Hayward, CA) and normalized per protein concentration using a Micro BCA Protein Assay kit (Thermo Scientific). …
Nucleophosmin, a critical Bax cofactor in ischemia-induced cell death
Zhiyong Wang, Jonathan M Gall, Ramon Bonegio, Andrea Havasi, Katarina Illanes, John H Schwartz, Steven C Borkan
Cell. Biol. (6.2), 2013-05-24, 33, 1916-24
… digitonin (8 μg/ml). Cytosolic proteins were extracted from renal cortical samples using a commercially available tissue protein extraction kit (BioChainInstitute, Newark, CA) by following the manufacturer’s instructions. Briefly, 50 to …
Functional proteomics reveals the protective effects of saffron ethanolic extract on hepatic ischemia-reperfusion injury
Tai-Long Pan, Tung-Ho Wu, Pei-Wen Wang, Yann-Lii Leu, Nardnisa Sintupisut, Chun-Hsun Huang, Fang-Rong Chang, Yang-Chang Wu
Proteomics (4.8), 2013-05-21, ,
… According to the manufacturer’s instructions, the liver tissue was homogenized in ice-cold buffer C (CNM compartment protein extraction kit, BioChainInstitute). Samples from cytosol and membrane fractions were denatured and then subjected to SDS-PAGE. The translocation of …
Baicalin ameliorates neuropathic pain by suppressing HDAC1 expression in the spinal cord of spinal nerve ligation rats
Chen-Hwan Cherng, Kwong-Chiu Lee, Chih-Cheng Chien, Kuang-Yi Chou, Yu-Che Cheng, Shih-Tai Hsin, Sing-Ong Lee, Ching-Hui Shen, Ru-Yin Tsai, Chih-Shung Wong
Formos. Med. Assoc. (1.1), 2013-05-15, ,
… The dorsal part of the ipsilateral spinal cord was fractionated into cytosolic, membrane, and nuclear fractions using a cytoplasmic, nuclear, and membrane (CNM) compartment protein extraction kit as recommended by the manufacturer (BioChainInstitute Inc., Hayward, CA, USA …
Proteasome dysfunction mediates obesity-induced endoplasmic reticulum stress and insulin resistance in the liver
Toshiki Otoda, Toshinari Takamura, Hirofumi Misu, Tsuguhito Ota, Shigeo Murata, Hiroto Hayashi, Hiroaki Takayama, Akihiro Kikuchi, Takehiro Kanamori, Kosuke R Shima, Fei Lan, Takashi Takeda, Seiichiro Kurita, Kazuhide Ishikura, Yuki Kita, Kaito Iwayama, Ken-ichiro Kato, Masafumi Uno, Yumie Takeshita, Miyuki Yamamoto, Kunpei Tokuyama, Shoichi Iseki, Keiji Tanaka, Shuichi Kaneko
Diabetes (8.9), 2013-03-22, 62, 811-24
… Nuclear matrix and cell membrane fractions were extracted from whole cell lysates by using the CNMCS compartmental protein extraction kit (BioChain Institute) according to the manufacturer’s protocol. Electron microscopy. …
Expression of Notch receptor and its target gene Hes-1 in bone marrow CD34+ cells from patients with psoriasis
Guohua Yin, Ruixia Hou, Junqin Li, Jing Zhang, Xinhua Li, Kaiming Zhang
Dermatology (Basel) (2.7), 2012-12-04, 225, 147-53
… goto top of outline Extraction of Total RNA and Protein from CD34+ Cells. The total RNA and protein of the same specimen were extracted simultaneously using the Dr. P kit (BioChain; Hayward, Hayward, Calif., USA) following the manufacturer’s recommendations. …
Eight new mutations and the expanding phenotype variability in muscular dystrophy caused by ANO5
S Penttilä, J Palmio, T Suominen, O Raheem, A Evilä, N Muelas Gomez, G Tasca, L B Waddell, N F Clarke, A Barboi, P Hackman, B Udd
Neurology (8), 2012-03-20, 78, 897-903
… RNA was extracted from muscle tissue with a Dr. P Kit (BioChain, Hayward, CA) for Finnish patients or by a modified TRIzol extraction protocol (TRI Reagent; Molecular Research Center, Cincinnati, OH) for Australian patients. …
Genotoxic agents promote the nuclear accumulation of annexin A2: role of annexin A2 in mitigating DNA damage
Patricia A Madureira, Richard Hill, Patrick W K Lee, David M Waisman
PLoS ONE (4.4), 2012-12-11, 7, e50591
… Nuclear/Cytoplasmic/Membrane/CytoskeletonFractionation. For multi-compartmental fractionation of cells we used the CNMS compartment protein extraction kit (BioChainInstitute) according to the manufacturer’s instructions. …
Phthalates stimulate the epithelial to mesenchymal transition through an HDAC6-dependent mechanism in human breast epithelial stem cells
Tsung-Hua Hsieh, Cheng-Fang Tsai, Chia-Yi Hsu, Po-Lin Kuo, Jau-Nan Lee, Chee-Yin Chai, Ming-Feng Hou, Chia-Cheng Chang, Cheng-Yu Long, Ying-Chin Ko, Eing-Mei Tsai
Sci. 0, 2012-08-16, 128, 365-76
… Subcellular fractionation. To assess protein translocation, the nuclearand cytoplasmic fractions of R2d cells were isolated using the Compart mental Protein Extraction kit CNMCS (BioChain, Hayward, CA) according to the manufacturer’s protocol. …
Galectin-1 promotes lung cancer progression and chemoresistance by upregulating p38 MAPK, ERK, and cyclooxygenase-2
Ling-Yen Chung, Shye-Jye Tang, Guang-Huan Sun, Teh-Ying Chou, Tien-Shun Yeh, Sung-Liang Yu, Kuang-Hui Sun
Cancer Res. (7.3), 2012-08-01, 18, 4037-47
… secondary antibodies. The antibodies used in this study are shown in Supplementary Table S5. Cytoplasm and nucleus of cells were separated by using the CNMCS Compartmental Protein Extraction Kit (BioChain). Whole-cell …
Increased Cdk5/p35 activity in the dentate gyrus mediates depressive-like behaviour in rats
Wei-Li Zhu, Hai-Shui Shi, Shen-Jun Wang, Chun-Mei Xu, Wen-Gao Jiang, Xi Wang, Ping Wu, Qian-Qian Li, Zeng-Bo Ding, Lin Lu
J. Neuropsychopharmacol. (4.7), 2012-07-11, 15, 795-809
… Beyotime Biotechnology, China). The cytoplasmic and membrane fractions were sep- arated using the CNM (cytoplasmic, nuclear, mem- brane) Compartment Protein Extraction kit (K3012010, BioChainInstitute, USA). The protein …
Allosteric modulation of beta1 integrin function induces lung tissue repair
Rehab Aljamal-Naylor, Linda Wilson, Susan McIntyre, Fiona Rossi, Beth Harrison, Mark Marsden, David J Harrison
Adv Pharmacol Sci 0, 2012-04-16, 2012, 768720
… At the end of the stretch, the media was aspirated, and protein extracted from the cell layer was fractionated using the compartmental protein extraction kit (CNMCS, BioChain). Protein content was measured using the BCA methods. …
Amitriptyline attenuates astrocyte activation and morphine tolerance in rats: role of the PSD-95/NR1/nNOS/PKCγ signaling pathway
Ya-Ni Huang, Ru-Yin Tsai, Shinn-Long Lin, Chih-Cheng Chien, Chen-Hwan Cherng, Ching-Tang Wu, Chun-Chang Yeh, Chih-Shung Wong
Brain Res. (3.4), 2012-04-15, 229, 401-11
… fraction preparation, cytosolic and membrane fractions of the dorsal portion of the lumbar spinal cord enlargement were prepared using a Cytoplasmic, Nuclear, and Membrane compartment protein extraction kit as recommended by the manufacturer (BioChainInstitute, Inc …
Ultra-low-dose naloxone enhances the antinociceptive effect of morphine in PTX-treated rats: regulation on global histone methylation
Ru-Yin Tsai, Ching-Hui Shen, Yu-Pin Feng, Chih-Cheng Chien, Sing-Ong Lee, Wei-Yuan Tsai, Yaoh-Shiang Lin, Chih-Shung Wong
Acta Anaesthesiol Taiwan 0, 2012-09-02, 50, 106-11
… The dorsal part of the spinal cord was fractionated into cytosolic, nuclear, and membrane fractions using a cytoplasmic, nuclear, and membrane compartment protein extraction as recommended by the manufacturer (BioChainInstitute, Inc., Hayward, CA, USA) and stored at …
Explore the Molecular Mechanism of Apoptosis Induced by Tanshinone IIA on Activated Rat Hepatic Stellate Cells
Tai-Long Pan, Pei-Wen Wang
Evid Based Complement Alternat Med (3), 2012-01-24, 2012, 734987
… previously [28]. Briefly, the 1 × 10 8 HSC-T6 cells with or without Tan IIA were suspended in ice-cold buffer C (CNM compartment protein extraction kit, BioChainInstitute) according to the manufacturer’s instructions. For cytoplasmic …
Anh-Nguyet T. Nguyen, Kyle Jansson, Gladis Sánchez, Madhulika Sharma, Gail A. Reif, Darren P. Wallace, and Gustavo Blanco
Reduction of intestinal mucosal immune function in heat-stressed rats and bacterial translocation
Xiaoxi Liu, Huanrong Li, An Lu, Yougang Zhong, Xiaolin Hou, Ning Wang, Dan Jia, Junlan Zan, Hong Zhao, Jianqin Xu, Fenghua Liu
Int J Hyperthermia (2.9), 2012-11-16, 28, 756-65
… Thus, protein from rat jejunum was extracted by a total protein extraction kit (K3011010, BioChain, San Francisco, CA, USA). The concentration of extracted protein was quantified by a BCA protein assay kit (23225, Pierce, Thermo Scientific, Rockford, IL, USA). …
Resveratrol regulates N-methyl-D-aspartate receptor expression and suppresses neuroinflammation in morphine-tolerant rats
Ru-Yin Tsai, Kuang-Yi Chou, Ching-Hui Shen, Chih-Cheng Chien, Wei-Yuan Tsai, Ya-Ni Huang, Pao-Luh Tao, Yaoh-Shiang Lin, Chih-Shung Wong
Analg. (3.3), 2012-10-24, 115, 944-52
… The dorsal portion of the lumbar spinal cords was fractionated into cytosolic, membrane, and nuclear fractions using a cytoplasmic, nuclear, and membrane compartment protein extraction kit, as recommended by the manufacturer (BioChainInstitute, Inc., Hayward, CA). …
miR-548c-5p inhibits proliferation and migration and promotes apoptosis in CD90(+) HepG2 cells
Lin Fang, Hai-Bing Zhang, Hua Li, Yong Fu, Guang-Shun Yang
Radiol Oncol (2), 2012-09-18, 46, 233-41
… and caspase-8). Caspase-3, bcl-2 and bcl-xl protein expression Total protein was extracted at 48 h after the trans- fection with Total Protein Extraction Kit (BioChain, USA) according to manufacturer’s instructions. In brief, 100 …
Carnosic acid (CA) prevents lipid accumulation in hepatocytes through the EGFR/MAPK pathway
Ting Wang, Yasuhiro Takikawa, Takahito Tabuchi, Takumi Satoh, Kunio Kosaka, Kazuyuki Suzuki
J. Gastroenterol. (3.6), 2012-07-19, 47, 805-13
… tractant protein 1 (MCP-1). Western blot analysis Total protein was isolated from liver tissue or HepG2 cells by a total protein extraction kit purchased from BioChain Institute, Inc. (Hayward, CA, USA). The nuclear fraction was …
Sandrine Vitry, Julie Bruyère, Michaël Hocquemiller, Stéphanie Bigou, Jérôme Ausseil, Marie-Anne Colle, Marie-Christine Prévost, and Jean Michel Heard Storage Vesicles in Neurons Are Related to Golgi Complex Alterations in Mucopolysaccharidosis IIIB J. Pathol., Dec 2010; 177: 2984 – 2999.
…. …NaCl, 50 mmol/L Tris pH7.8, protease inhibitors). Membrane proteins were extracted with a CNMCS Compartmental extraction kit (Biochain, Hayward, US) according to manufacturer recommendations. Endo-H (Biolabs, Beverly, US) and peptide N-glycosidase F (PNGase… …
Chi-Wen Lo, Min-Wei Chen, Michael Hsiao, Shiuan Wang, Chi-An Chen, Sheng-Mou Hsiao, Jeng-Shou Chang, Tsung-Ching Lai, Stefan Rose-John, Min-Liang Kuo, and Lin-Hung Wei IL-6 Trans-Signaling in Formation and Progression of Malignant Ascites in Ovarian Cancer Cancer Res., Jan 2011; 71: 424 – 434.
… …Tyr 685; ECM Biosciences). Cytoplasmic VE-cadherin protein was prepared by the CNMCS compartmental protein extraction kit (BioChain). Cytotoxicity assay Cell survival was determined using the MTT assay (Sigma). Assay of chemotactic endothelial cell… …link
Weijing Cai, Massimo Torreggiani, Li Zhu, Xue Chen, John Cijiang He, Gary E. Striker, and Helen Vlassara AGER1 regulates endothelial cell NADPH oxidase-dependent oxidant stress via PKC- : implications for vascular disease Am J Physiol Cell Physiol, Mar 2010; 298: C624 – C634.
… …membranes and nuclear proteins were prepared from ECV304, HAEC cells, and mouse aortic segments using a kit purchased from BioChainInstitute (Hayward, CA). Cells were centrifuged, and the pellet was washed with PBS 3, and then ice-cold buffer C (HEPES pH…
Ali Ö. Yildirim, Vandana Muyal, Gerrit John, Bernd Müller, Carola Seifart, Michael Kasper, and Heinz Fehrenbach Palifermin Induces Alveolar Maintenance Programs in Emphysematous Mice Am. J. Respir. Crit. Care Med., Apr 2010; 181: 705 – 717.
… …details.) Protein Isolation and Immunoblotting Protein was extracted from lung tissue using total protein extraction kit (Biochain Institute, Hayward, CA). Protein concentrations were measured using BCA protein assay kit (Pierce, Rockford, IL). After fragmentation… …
Ali O Yildirim, Vandana Muyal, Gerrit John, Bernd Mueller, Carola Seifart, Michael Kasper, and Heinz Fehrenbach Palifermin Induces Alveolar Maintenance Programs in Emphysematous Mice J. Respir. Crit. Care Med., Dec 2009; 10.1164/rccm.200804-573OC.
… …extracted from lung tissue using total protein extraction kit (BiochainInstitute, Hayward, USA). Protein concentrations were measured using BCATM protein assay kit (Pierce, Rockford, USA). After fragmentation…extracted from lung tissue using total protein extraction kit (Biochain Institute, Hayward, USA) according to the manufacturer’s protocol… …
Guomin Jiang, Yan Ke, Deming Sun, Hao Li, Mark Ihnen, Marcia M. Jumblatt, Gary Foulks, Yali Wang, Yang Bian, Henry J. Kaplan, and Hui Shao A New Model of Experimental Autoimmune Keratoconjunctivitis Sicca (KCS) Induced in Lewis Rat by the Autoantigen Klk1b22 Ophthalmol. Vis. Sci., May 2009; 50: 2245 – 2254.
… …glands and salivary glands of B6 mice by homogenization and sonication following the method of a total protein extraction kit (BioChainInstitute, Inc., Hayward, CA). Briefly, the glands were isolated from PBS perfused mice, cut into small pieces, and incubated… …
Wu, S. Chang, Y. Chen, Q. Xia, M. Forbes, and J. C. Tsai Erythropoietin Receptor Plays a Role in the Cell Differentiation of Retina and Lens During Eye Development
Invest. Ophthalmol. Vis. Sci., Apr 2008; 49: 3078.
… …without retina). All collected tissues were stored at -70C for tissue protein preparation using a whole protein extract kit (Biochain Institute, Inc.). The total protein lysate (15microl) was subject to 12% SDS-PAGE followed by western blotting procedure with… …
Jennifer E. Bruin, Hertzel C. Gerstein, Katherine M. Morrison, and Alison C. Holloway Increased Pancreatic Beta-Cell Apoptosis following Fetal and Neonatal Exposure to Nicotine Is Mediated via the Mitochondria Sci., Jun 2008; 103: 362 – 370.
… …Science, Laval, PQ). To separate mitochondrial and cytosolic fractions, the Compartmental Protein Extraction Kit (K3013010; Biochain Institute, Inc., Hayward, CA) was used according to manufacturer’s instructions. For Western blots of whole pancreas homogenates… …
Yingqiu Xie, Kexin Xu, Douglas E. Linn, Xi Yang, Zhiyong Guo, Hermela Shimelis, Takeo Nakanishi, Douglas D. Ross, Hegang Chen, Ladan Fazli, Martin E. Gleave, and Yun Qiu The 44-kDa Pim-1 Kinase Phosphorylates BCRP/ABCG2 and Thereby Promotes Its Multimerization and Drug-resistant Activity in Human Prostate Cancer Cells Biol. Chem., Feb 2008; 283: 3349 – 3356.
… …for three times at 4 C with the lysis buffer. Cell fractionation was carried out using compartmental protein extraction kit (BioChain, Inc.). Immunoblotting was performed as described previously (24). Briefly, blots were incubated with primary antibodies, 1… … Ref. link
Emily I. Chen, Johannes Hewel, Joseph S. Krueger, Claire Tiraby, Martin R. Weber, Anastasia Kralli, Katja Becker, John R. Yates, III, and Brunhilde Felding-Habermann Adaptation of Energy Metabolism in Breast Cancer Brain Metastases
Cancer Res., Feb 2007; 67: 1472 – 1486.
… …extracts were prepared with the TotalProteinExtraction kit (BioChain, Hayward, CA) and protein concentration was determined…extracts were prepared with the TotalProteinExtraction kit (BioChain) and protein concentrations determined by BCA assay (Pierce… … link
Pinthus JH, Bryskin I, Trachtenberg J, Lu JP, Singh G, Fridman E, Wilson BC. Androgen Induces Adaptation to Oxidative Stress in Prostate Cancer: Implications for Treatment with Radiation Therapy.
2007 Jan; 9(1): 68-80.
… …Centrifuging at 12,000g for 15 minutes separated insoluble materials. Supernatants were dissolved in Laemmli sample buffer. Protein purification from tumor tissues was performed using a protein extraction kit (Biochain Institute, Inc., Greenland, NH)…. … Ref. link
Chun-Chun Li, Tsai-Chen Chiang, Tsung-Sheng Wu, Gustavo Pacheco-Rodriguez, Joel Moss, and Fang-Jen S. Lee ARL4D Recruits Cytohesin-2/ARNO to Modulate Actin Remodeling
Mol. Biol. Cell, Sep 2007; 10.1091/mbc.E07-02-0149.
… …DSP and then the cytosol and membrane fractions of COS-7 cells were prepared using a CNM compartment protein extraction kit (BioChain Institute Inc., Hayward, CA) according to the manufacturer’s instructions. Pull-down Assay for ARF6-GTP The activation levels… … Ref. link
Kasie K. Cole-Edwards, Alberto E. Musto, and Nicolas G. Bazan c-Jun N-Terminal Kinase Activation Responses Induced by Hippocampal Kindling Are Mediated by Reactive Astrocytes
Neurosci., Aug 2006; 26: 8295 – 8304.
… …Alternatively, to separate brain tissue into cytosolic, membrane, and nuclear protein fractions, a compartmental extraction kit from BioChain(Hayward, CA) was used. Tissue was homogenized in a Dounce homogenizer with five strokes of the loose-fitting pestle and manipulated… … Ref. link
Brian J. Siroky, William B. Ferguson, Amanda L. Fuson, Yi Xie, Attila Fintha, Peter Komlosi, Bradley K. Yoder, Erik M. Schwiebert, Lisa M. Guay-Woodford, and P. Darwin Bell Loss of primary cilia results in deregulated and unabated apical calcium entry in ARPKD collecting duct cells
Am J Physiol Renal Physiol, Jun 2006; 290: F1320 – F1328.
… …PC2 antibody. Cytosolic and membrane protein fractions were isolated using the CNM Compartment Protein Extraction Kit (BioChain). Proteins were separated using polyacrylamide electrophoresis and transferred onto PVDF membranes. Proteins labeled with… …
Jia-Perng Chen, Hsin-Lin Lu, Szu-Liang Lai, Gabriele S. Campanella, Jui-Ming Sung, Mei-Yi Lu, Betty A. Wu-Hsieh, Yi-Ling Lin, Thomas E. Lane, Andrew D. Luster, and Fang Liao Dengue Virus Induces Expression of CXC Chemokine Ligand 10/IFN–Inducible Protein 10, Which Competitively Inhibits Viral Binding to Cell Surface Heparan Sulfate
Immunol., Sep 2006; 177: 3185 – 3192.
… …the reference were approximately equal. ELISA Livers were weighed and homogenized on ice in protein extraction buffer (BioChainInstitute) using a tapered tissue grinder (Wheaton Science Products). The resultant homogenates were centrifuged at 15,000… … Ref. link
Chang, R. Yamada, A. Suzuki, Y. Kochi, T. Sawada, and K. Yamamoto Citrullination of fibronectin in rheumatoid arthritis synovial tissue
Rheumatology, Nov 2005; 44: 1374 – 1382.
… …the Total Protein Extraction Kit (Biochain, USA) according to the manufacturers…prostate and thymus adenocarcinoma (BioChain). Fn in the total proteins was precipitated…the Total Protein Extraction Kit (Biochain, USA) according to the manufacturer’s… …
Emily I. Chen, Laurence Florens, Fumiko T. Axelrod, Edward Monosov, Carlos F. Barbas III, John R. Yates III, Brunhilde Felding-Habermann, and Jeffrey W. Smith Maspin alters the carcinoma proteome
FASEB J, Apr 2005; 10.1096/fj.04-2970fje.
… …using TotalProteinExtraction kit (BioChain, Hayward, CA), and protein concentration…homogenized in the lysis buffer from the Biochain total protein extraction kit (Biochain). Cellular 20S proteasome activity… …
Chang, R. Yamada, A. Suzuki, T. Sawada, S. Yoshino, S. Tokuhiro, and K. Yamamoto Localization of peptidylarginine deiminase 4 (PADI4) and citrullinated protein in synovial tissue of rheumatoid arthritis
Rheumatology, Jan 2005; 44: 40 – 50.
… we purified total protein of RA synovial tissues and leucocytes using total protein extraction kits (Biochain). …
Christian Korn, Sebastian R. Scholz, Oleg Gimadutdinow, Rudi Lurz, Alfred Pingoud, and Gregor Meiss Interaction of DNA Fragmentation Factor (DFF) with DNA Reveals an Unprecedented Mechanism for Nuclease Inhibition and Suggests That DFF Can Be Activated in a DNA-bound State
Chem., Feb 2005; 280: 6005 – 6015.
… Endogenous DFF ?Non-detergent cell lysis was performed using the CNM compartmental protein extraction kit (BioChainInstitute, Inc.) according to the supplier’s recommendations. …
Neelam Sharma-Walia, Pramod P. Naranatt, Harinivas H. Krishnan, Ling Zeng, and Bala Chandran Kaposi’s Sarcoma-Associated Herpesvirus/Human Herpesvirus 8 Envelope Glycoprotein gB Induces the Integrin-Dependent Focal Adhesion Kinase-Src-Phosphatidylinositol 3-Kinase-Rho GTPase Signal Pathways and Cytoskeletal Rearrangements
Virol., Apr 2004; 78: 4207 – 4223.
… The CNM compartment protein extraction kit was obtained from the BiochainInstitute, Inc., Hayward, Calif. …
