- Pre-treatment of a single high-dose of atorvastatin provided cardioprotection in different ischaemia/reperfusion models via activating mitochondrial KATP channel
Z Zhao, W Cui, H Zhang, H Gao, X Li, Y Wang… – European journal of …, 2015 – Elsevier
… Volume 751, 15 March 2015, Pages 89–98. … Received 30 June 2014, Revised 18 January 2015,
Accepted 20 January 2015, Available online 30 January 2015. … Ator pre-conditioning, NAD +
content, a marker of mPTP opening, was measured with theNAD + Assay Kit (Biochain). … - Automated nucleic acid extraction from whole blood, B. subtilis, E. coli, and Rift Valley fever virus on a centrifugal microfluidic LabDisk
O Strohmeier, S Keil, B Kanat, P Patel, M Niedrig… – RSC Advances, 2015– pubs.rsc.org
… First published on the web 27th March 2015. … 27–29 For measurement of ethanol carry over from
washing buffers into the elution buffer, a commercially available kit for colorimetric determination
of ethanol concentration (Ethanol Assay Kit Z5030029, BioChain, USA) was used. … - Antcin K, an active triterpenoid from the fruiting bodies of basswood cultivated Antrodia cinnamomea, induces mitochondria and endoplasmic reticulum stress- …
CI Lai, YL Chu, CT Ho, YC Su, YH Kuo… – Journal of Traditional and …, 2015 – Elsevier
…Anti-LC3B antibody was purchased from GeneTex (Irvine, CA, USA). Adenosine
diphosphate/adenosine triphosphate (ADP/ATP) ratio assay kit and lactate dehydrogenase (LDH)
cytotoxicity assay kit were purchased from BioChain Institute (Hayward, CA, USA). … - L-Arginine administration attenuates airway inflammation by altering L-arginine metabolism in an NC/Nga mouse model of asthma
R Zhang, M Kubo, I Murakami, H Setiawan… – Journal of Clinical …, 2015 – jlc.jst.go.jp
…The samples were incubated in the presence of Arg and MnCl2 at 37°C for 60 min. The
amount of the reaction product urea was measured using a urea assay kit (BioChain Institute,
, Hayward, CA) according to the manufacturer’s instructions. … - Laser transfection with gold nanoparticles: current state and new particle structures as a perspective
S Kalies, L Gentemann… – SPIE …, 2015 – proceedings.spiedigitallibrary.org
…Before normalization, a control group which was only laser treated was subtracted. Additionally,
the cell viability was analyzed with a resazurin based assay (QBlue, Biochain) and normalized
to an untreated control. 3. RESULTS AND DISCUSSION 3.1. … - Plasmonic cell manipulation for biomedical and screening applications
D Heinemann, M Schomaker, S Kalies… – SPIE …, 2015 – proceedings.spiedigitallibrary.org
…O O O O O I I I I I Sigma-Aldrich, United States) in PBS. The analysis was conducted with a FACS
Calibur flow cytometer (BD Bioscience, Germany). The cell viability after caspase 3 delivery was
evaluated 24 h after GNOME laser transfection using the QBlue kit (BioChain, USA). … - Perspectives in nanostructure assisted laser manipulation of mammalian cells
D Heinemann, M Schomaker, S Kalies… – SPIE …, 2015 – proceedings.spiedigitallibrary.org
…The viability was tested using the resazurin based QBlue viability assay (BioChain, USA) after
laser treatment. For this, 10% (v/v) QBlue were added to the culture medium and then incubated
for 1 to 2 h. Then, the resorufin fluorescence was determined at EX570/EM600. … - Pre-treatment of a single High-dose of atorvastatin provided cardioprotection in different ischaemia/reperfusion models via activating mitochondrial KATP channel
Z Zhao, W Cui, H Zhang, H Gao, X Li, Y Wang… – European Journal of …, 2015 – Elsevier
…investigate the relationship between mitochondrial KATP channels (mitoK ATP channels) and
mitochondrial permeability transition pores (mPTPs) implicated in Ator pre-conditioning, NAD +
content, a marker of mPTP opening, was measured with the NAD + Assay Kit (Biochain … - HAS Murad, HA Saleh, GS Abdulaziz… – International Journal of …, 2015 – Springer
Effect of metformin and pioglitazone on β-catenin and biochemical markers in sitagliptin-induced pancreatitis in diabetic rats
…The manufacturers of the kits are as follows: glucose (Sigma), cholesterol (BioChain, Hayward,
CA, USA), TG (Xpress Bio, Thurmont, MD, USA), amylase (Abnova, Neihu District, Taipei, Tai-
wan), MDA (Cell Biolabs, Inc., San Diego, CA, USA), - T Hayashi, Y Asano, Y Shintani… – Proceedings of the …, 2015 – National Acad Sciences
Higd1a is a positive regulator of cytochrome c oxidase
…for 30 min, before Raman measurements. Measurement of CcO Activity. CcO activity
was measured spectrophotomet- rically (Shimazu, UV-2450) using a cytochrome c
oxidase activity kit (Bio– chain). - CHIP is an Essential Determinant of Neuronal Mitochondrial Stress Signaling
AM Palubinsky, JN Stankowski… – Antioxidants and …, 2015 – online.liebertpub.com
… Detection Kit (Millipore, S7150) and an ATP/ADP Ratio Assay Kit (Z5030042, BioChain). Reagents
and materials required for the biotin-avidin-capture methodology were … Genotyping is performed
by PCR with DNA from tail clippings using primers for the CHIP allele - TREATMENT OF MUCOSITIS WITH IMMUNOGLOBULIN
C Aebi, SC Lueer, A Schaub… – US Patent …, 2015 – freepatentsonline.com
…Cell viability was ascertained morphologically, by trypan blue exclusion, and a commercial LDH
assay (BioChain Institute, Inc., Hayward, Calif.). … layer thickness, number of basal cells in mucosa,
histological quantification of the proliferation marker KI-67, mRNA transcripts - Endoplasmic Reticulum Stress-Activated Glycogen Synthase Kinase 3β Aggravates Liver Inflammation and Hepatotoxicity in Mice with Acute Liver Failure
F Ren, L Zhou, X Zhang, T Wen, H Shi, B Xie, Z Li… – Inflammation, 2015 – Springer
…Both the mRNA and liver tissues were stored at −75 °C for later analysis. … primary hepatocytes
also stimulated with TNF-α (20 ng/ml)/ActD (1 nM) in the absence or presence of 4-PBA (1 mM)
for 12 h. Cell apoptosis was evaluated at 12 h LDH assays (BioChain Institute, Hayward - The glycaemic and C-peptide responses of foods rich in dietary fibre from oat, buckwheat and lingonberry
Susanna Rokka, Elise Ketoja, Eila Järvenpää, Raija Tahvonen
Int J Food Sci Nutr (0.8), 2013-07-09, 64, 528-34
… Data table, Table II. The carbohydrate compositions of test foods (g/100 g) and the carbohydrate content of each test food serving (g). The glucose level of serum was measured using quantitative colorimetric glucose assay kit (BioChain, Hayward, CA, USA) at 650 nm. … - Amelioration of ethanol-induced liver injury in rats by nanogold flakes
Ya-Ling Chen, Hsiang-Chi Peng, Shan-Wen Tan, Cheng-Yuh Tsai, Yi-Huei Huang, Hao-Yu Wu, Suh-Ching Yang
Alcohol (2.4), 2013-07-02, ,
… Measurements and analytical procedures. Blood alcohol concentration. Blood samples were collected 2 h after the ethanol liquid diet feeding. The blood alcohol concentration was determined using an ethanol assay kit (BioChain, Hayward, CA, USA). … - Nucleoside Reverse Transcriptase Inhibitors Induce a Mitophagy-Associated Endothelial Cytotoxicity That Is Reversed by Coenzyme Q10 Cotreatment
Stephen Y Xue, Valeria Y Hebert, Danicia M Hayes, Corie N Robinson, Mitzi Glover, Tammy R Dugas
Sci. 0, 2013-06-04, ,
… Using a kit purchased from BioChain(Hayward, CA), complex IV activity was determined by monitoring the decrease in absorbance at 550nm produced by the cytochrome c oxidase-mediated oxidation of ferrocytochrome c to ferricytochrome c. … - Comparison of Contrast Agents for Atherosclerosis Imaging Using Cultured Macrophages: FDG Versus Ultrasmall Superparamagnetic Iron Oxide
Tomoko Satomi, Mikako Ogawa, Ikuo Mori, Seigo Ishino, Kazuki Kubo, Yasuhiro Magata, Tomoyuki Nishimoto
Nucl. Med. (7), 2013-06-04, 54, 999-1004
… The iron contents of cell lysates were measured using the Iron Assay Kit (BioChain). Cellular protein concentration was measured using the DC Protein Assay Kit. The USPIO accumulation was expressed as cellular iron content per milligram of cell protein. … - Evaluation of polypropylene hollow-fiber prototype bioreactor for bioartificial liver
Anwar Azad Palakkan, Deepa K Raj, Jose Rojan, Sajin Raj R G, P R Anil Kumar, C V Muraleedharan, T V Kumary
Tissue Eng Part A 0, 2013-05-27, 19, 1056-66
… Urea estimation Urea production by cells in a prototype bioreactor was estimated using an urea assay kit (BioChain) as per the manufacturer’s instructions. The medium collected on 1, 3, 5, and 7 days was used for analysis. … - Evaluation of the potential therapeutic role of a new generation of vitamin D analog, MART-10, in human pancreatic cancer cells in vitro and in vivo
Kun-Chun Chiang, Chun-Nan Yeh, Jun-Te Hsu, Ta-sen Yeh, Yi-yin Jan, Chun-Te Wu, Huang-Yang Chen, Shyh-Chuan Jwo, Masashi Takano, Atsushi Kittaka, Horng-Heng Juang, Tai C Chen
Cell Cycle 0, 2013-04-15, 12, 1316-25
… week for 3 wk. The body weight, serum calcium and tumor volume were measured during the experimental period. Serum calcium was measured by calcium assay kit (#Z5030014, BioChain). Tumor volumes were calculated … - Arterial klotho expression and FGF23 effects on vascular calcification and function
Karolina Lindberg, Hannes Olauson, Risul Amin, Arvind Ponnusamy, Regina Goetz, Rebecca F Taylor, Moosa Mohammadi, Ann Canfield, Karolina Kublickiene, Tobias E Larsson
PLoS ONE (4.4), 2013-04-11, 8, e60658
… Sequences of the primers used for genotyping are listed in Table S1. Serum biochemistries. Serum calcium, phosphate and creatinine were measured using quantitative colorimetric assay kits (BioAssay/BioChain, Hayward, CA, US). … - Gold nanoparticle mediated laser transfection for efficient siRNA mediated gene knock down
Dag Heinemann, Markus Schomaker, Stefan Kalies, Maximilian Schieck, Regina Carlson, Hugo Murua Escobar, Tammo Ripken, Heiko Meyer, Alexander Heisterkamp
PLoS ONE (4.4), 2013-03-28, 8, e58604
… times. In order to determine the viability, 10% (v/v) of the QBlue viability assay kit (BioChain, Newark, USA), a resazurin based, fluorometric metabolism assay, were added to the culture medium and incubated for one hour. Delivery .. - Effects of herpes simplex virus vector-mediated enkephalin gene therapy on bladder overactivity and nociception
Hitoshi Yokoyama, Tomohiko Oguchi, William F Goins, James R Goss, Osamu Nishizawa, William C de Groat, Darren Wolfe, David M Krisky, Joseph C Glorioso, Naoki Yoshimura
Gene Ther. (4.8), 2013-02-20, 24, 170-80
… Rocky Hill, NJ). Urinary creatinine was also measured in each sample by the Creatinine Assay Kit (BioChain, Hayward, CA), and the levels were used to standardize urinary cytokine levels. Cystometrograms Two weeks after … - Down-regulation of NAMPT expression by miR-182 is involved in Tat-induced HIV-1 long terminal repeat (LTR) transactivation
Xin-Yu Chen, Hong-Sheng Zhang, Tong-Chao Wu, Wei-Wei Sang, Zheng Ruan
J. Biochem. Cell Biol. (5), 2013-02-18, 45, 292-8
… 2.8. NAD + assay. The intracellular NAD + levels were measured using a BioChainNAD + /NADH assay kit according to manufacturer’s instructions (BioChain, Hayward, CA). NAD + levels were calculated according to the equivalent protein quantity (μg protein) per data point. … - Dielectric characterization of hepatocytes in suspension and embedded into two different polymeric scaffolds
M Massimi, A Stampella, L Conti Devirgiliis, G Rizzitelli, A Barbetta, M Dentini, C Cametti
Colloids Surf B Biointerfaces (2.8), 2013-02-01, 102, 700-7
… secretion assay. Human albumin was used to establish a calibration curve. Urea concentrations were determined with a urea assay kit (BioChainInstitute, Hayward, CA), in accordance with the manufactures’s instructions. The levels … - Transient receptor potential melastatin 4 channel controls calcium signals and dental follicle stem cell differentiation
Piper Nelson, Tran Doan Ngoc Tran, Hanjie Zhang, Olga Zolochevska, Marxa Figueiredo, Ji-Ming Feng, Dina L Gutierrez, Rui Xiao, Shaomian Yao, Arthur Penn, Li-Jun Yang, Henrique Cheng
Stem Cells (7.9), 2013-01-21, 31, 167-77
… Alkaline Phosphatase Activity. Quantification of alkaline phosphatase (ALP) enzyme activity at different time points during osteogenesis was made with an ALP assay kit (BioChain, Newark, CA) according to the manufacturer’s instructions. … - Nanoporous fibers of type-I collagen coated poly (l-lactic acid) for enhancing primary hepatocyte growth and function
Wang, Feng, Leach, Wu
Journal of Materials … 0, , ,
… Laboratories Inc, Montgomery, USA). Urea synthesis by hepatocytes incubated in fresh medium containing 2 mM NH 4 Cl for 90 min was measured with the Urea Nitrogen kit (BioChainInstitute Inc, Boerne, USA). Cytochrome P-450 … - Oxidative stress biomarkers versus Magnetic Resonance Imaging (MRI) assessment in patients with tempromandibular disorders
Fleifel, AlKhiary
Journal of American Science 0, , ,
… GSH Assay: To determine intracellular levels of reduced glutathione (GSH), cells were lysed with protein lysis buffer. GSH levels were determined using the Glutathione assay kit (BioChain) following the manufacturer’s instructions. … - The Effect on a New Sintered Calcium Sulfate Ceramic to Osteoprogenitor MC 3T3-E1
Wu, Liu, Chen, Kuo
Advanced Materials … 0, , ,
… Alkaline Phosphatase Assay Kit (BioChainZ5030033) was used to measure ALP activity with p-nitrophenyl phosphate as substrate directly in biological samples. … Calcium Assay Kit (BioChain, Z5030014) was used to measure the calcium concentration. … - Intrapulmonary penetration of ceftolozane/tazobactam and piperacillin/tazobactam in healthy adult subjects
Gurudatt Chandorkar, Jennifer A Huntington, Mark H Gotfried, Keith A Rodvold, Obiamiwe Umeh
Antimicrob. Chemother. (4.7), 2012-10-18, 67, 2463-9
… The lower limit of detection for all three drugs in BAL was 1.00 ng/mL. Urea concentrations in plasma and BAL were performed at MicroConstants, Inc. (San Diego, CA, USA) using a colorimetric technique (Urea Assay Kit Z5030016, BioChain, Hayward, CA, USA). … - Comparison of plasma, epithelial lining fluid, and alveolar macrophage concentrations of solithromycin (CEM-101) in healthy adult subjects
Keith A Rodvold, Mark H Gotfried, J Gordon Still, Kay Clark, Prabhavathi Fernandes
Agents Chemother. (4.7), 2012-10-13, 56, 5076-81
… The lower limit of detection for solithromycin in cell pellet samples was 1.00 ng/ml. Urea concentration determination.Concentrations of urea in plasma and BAL fluid supernatant were determined using the urea assay kit fromBioChain(Hayward, CA) at MicroConstants, Inc. … - Copper-trafficking efficacy of copper-pyruvaldehyde bis(N4- methylthiosemicarbazone) on the macular mouse, an animal model of Menkes disease
Mitsutoshi Munakata, Hiroko Kodama, Chie Fujisawa, Tomoko Hiroki, Kazuhiko Kimura, Mika Watanabe, Masazumi Nishikawa, Shigeru Tsuchiya
Res. (2.8), 2012-09-16, 72, 270-6
… and kept at −80 °C. A small amount (~6 mg) of each mixed paste was separately pretreated in cooled n-dodecyl β-D-maltoside buffer for 15 min, and CO activity was measured using a spectrophotometric method (cytochrome c oxidase activity assay kit; BioChain, Hayward, CA … - Primary human hepatocytes from metabolic-disordered children recreate highly differentiated liver-tissue-like spheroids on alginate scaffolds
Jeanette Bierwolf, Marc Lutgehetmann, Steffen Deichmann, Johannes Erbes, Tassilo Volz, Maura Dandri, Smadar Cohen, Bjoern Nashan, Joerg-Matthias Pollok
Tissue Eng Part A 0, 2012-07-17, 18, 1443-53
… background. A positive and a high-positive control were moreover analyzed as quality control. Urea assay Urea production was determined by a quantitative colorimetric urea assay kit from BioChainInstitute (Hayward, CA). For … - CD73-generated adenosine promotes osteoblast differentiation
Masahide Takedachi, Hiroyuki Oohara, Brenda J Smith, Mitsuyoshi Iyama, Mariko Kobashi, Kenichiro Maeda, Courtney L Long, Mary B Humphrey, Barbara J Stoecker, Satoru Toyosawa, Linda F Thompson, Shinya Murakami
Cell. Physiol. (4), 2012-06-27, 227, 2622-31
… Serum inorganic phosphate was determined by the improved Malachite Green method utilizing the malachite green dye and molybdate provided by the Phosphate assay kit (BioChain, Hayward, CA). Reverse transcription (RT)-PCR. … - In vivo clearance and toxicity of monodisperse iron oxide nanocrystals
Luo Gu, Ronnie H Fang, Michael J Sailor, Ji-Ho Park
ACS Nano (9.9), 2012-06-26, 6, 4947-54
… The iron concentration of the serum sample was analyzed using an iron assay kit (BioChainInstitute, Inc.), and the blood half-life was calculated by fitting the absorbance data to a single-exponential equation using a one-compartment open pharmacokinetic model(28) (mean … - Platelet-rich fibrin increases cell attachment, proliferation and collagen-related protein expression of human osteoblasts
C-L Wu, S-S Lee, C-H Tsai, K-H Lu, J-H Zhao, Y-C Chang
Aust Dent J (1.5), 2012-06-25, 57, 207-12
… The resulting cell suspensions were placed in 96-well plates in 100 μl of DMEM together with 10 μl of a working solution containing WST-1 Cell Proliferation Assay Kit (BioChainInstitute, Hayward, CA, USA) and incubated for 2 hours at 37 °C. The absorbances of the coloured … - Chemokine and free fatty acid levels in insulin-resistant state of successful pregnancy: a preliminary observation
Katsuhiko Naruse, Taketoshi Noguchi, Toshiyuki Sado, Taihei Tsunemi, Hiroshi Shigetomi, Seiji Kanayama, Juria Akasaka, Natsuki Koike, Hidekazu Oi, Hiroshi Kobayashi
Mediators Inflamm. (2.1), 2012-04-12, 2012, 432575
… The lower limit of detection was 2 μM. To estimate alterations in unbound FFA, the data were corrected with serum albumin concentrations (FFA (μM)/albumin (g/dL)) by using the BCG albumin assay kit (BioChain, Hayward, CA). … - Deletion of the Cl-/HCO3- exchanger pendrin downregulates calcium-absorbing proteins in the kidney and causes calcium wasting
Sharon Barone, Hassane Amlal, Jie Xu, Manoocher Soleimani
Dial. Transplant. (3.6), 2012-04-02, 27, 1368-79
… water. Food intake, water intake and urine volume were measured daily. Urine was collected under mineral oil. Urine calcium concentration was measured via a Calcium Assay Kit (BioChainInstitute, Hayward, CA). Serum Ca … - Modulatory effect of resveratrol on SIRT1, SIRT3, SIRT4, PGC1α and NAMPT gene expression profiles in wild-type adult zebrafish liver
Helena Schirmer, Talita Carneiro Brandão Pereira, Eduardo Pacheco Rico, Denis Broock Rosemberg, Carla Denise Bonan, Maurício Reis Bogo, André Arigony Souto
Biol. Rep. (1.9), 2012-03-03, 39, 3281-9
… l). NAD? and NADH concentrations The coenzymes were measured using the NAD?/NADH colorimetric assay kit (BioChainHayward, CA, USA-LGC Biotechnology) in accordance with the manufacturer’s instructions. Frozen … - YanFei Qi, Vinayak Shenoy, Fong Wong, Hongwei Li, Aqeela Afzal, J. Mocco, Colin Sumners, Mohan K. Raizada, and Michael J. Katovich
Lentivirus-mediated overexpression of angiotensin-(1–7) attenuated ischaemia-induced cardiac pathophysiology
Exp Physiol, Sep 2011; 96: 863 – 874.
… …treatment. One hour after exposure to hypoxia, cell viability was tested using CellQuanti-BlueTM Cell Viability Assay Kits (BioChain, Hayward, CA, USA). Twenty-four hours after exposure to hypoxia, cell lysate was collected to isolate RNA. RNA isolation… … - Sharon Barone, Hassane Amlal, Jie Xu, and Manoocher Soleimani
Deletion of the Cl–/HCO3–exchanger pendrin downregulates calcium-absorbing proteins in the kidney and causes calcium wasting
Dial. Transplant., Aug 2011; 10.1093/ndt/gfr505.
… …were measured daily. Urine was collected under mineral oil. Urine calcium concentration was measured via a Calcium Assay Kit (BioChain Institute, Hayward, CA). Serum Ca2+ concentration was measured with an i-STAT-1 analyzer using i-STAT EG7+ cartridges (Abbott… … - Cornelia Bigler, Helmut Hopfer, Doris Danner, Monica Schaller, Michael J. Mihatsch, and Marten Trendelenburg
Anti-C1q autoantibodies do not correlate with the occurrence or severity of experimental lupus nephritis
Dial. Transplant., Apr 2011; 26: 1220 – 1228.
… …was measured by quantitative colorimetric determination at 510-nm using a commercially available assay (Creatinine Assay Kit, Biochain, Hayward, CA, USA). Antibody elution from kidneys The procedure was performed as described before [9]. In short, six kidneys… … - Juan Lucas Argueso, Marcelo F. Carazzolle, Piotr A. Mieczkowski, Fabiana M. Duarte, Osmar V.C. Netto, Silvia K. Missawa, Felipe Galzerani, Gustavo G.L. Costa, Ramon O. Vidal, Melline F. Noronha, Margaret Dominska, Maria G.S. Andrietta, Sílvio R. Andrietta, Anderson F. Cunha, Luiz H. Gomes, Flavio C.A. Tavares, André R. Alcarde, Fred S. Dietrich, John H. McCusker, Thomas D. Petes, and Gonçalo A.G. Pereira
Genome structure of a Saccharomyces cerevisiaestrain widely used in bioethanol production
Genome Res., Dec 2009; 19: 2258 – 2270.
… …the weight of the cell pellets. The supernatant was frozen and later used to determine the ethanol concentration using the BioChain Saccharide Removal and Ethanol Assay kits in sequence according to the manufacturer’s recommendations. Microarray and physical… … - Indira Padmalayam, Sumera Hasham, Uday Saxena, and Sivaram Pillarisetti
Lipoic Acid Synthase (LASY): A Novel Role in Inflammation, Mitochondrial Function, and Insulin Resistance
Diabetes, Mar 2009; 58: 600 – 608.
… …with protein lysis buffer 48 h after initiation of LASY knockdown. GSH levels were determined using the Glutathione assay kit (Biochain) following the manufacturers instructions. Data were expressed per milligram protein determined by the MicroBCA technique… … - Niti Puri and Paul A. Roche
Mast cells possess distinct secretory granule subsets whose exocytosis is regulated by different SNARE isoforms
PNAS, Feb 2008; 105: 2580 – 2585. …each treatment, the release of the cytosolic enzyme lactate dehydrogenase was determined by using LDH Cytotoxicity Assay kit (Biochain Institute, Inc.) according to instructions of the manufacturer. The percentage of LDH released after each treatment was calculated… … - Mark E. Lasbury, Salim Merali, Pamela J. Durant, Dennis Tschang, Chad A. Ray, and Chao-Hung Lee
Polyamine-mediated apoptosis of alveolar macrophages during Pneumocystis pneumonia
Biol. Chem., Feb 2007; 10.1074/jbc.M611686200.
… …1 by vacuum centrifugation) were incubated with the proprietary chromogenic, urea-complexing reagent of the Urea assay kit (Biochain, Hayward, CA). Samples were read at 520 nm after a 20-min incubation at room temperature. Sample absorbance was divided by… …
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