Nicotine up-regulates IL-8 expression in human gingival epithelial cells following stimulation with IL-1β or P. gingivalis lipopolysaccharide via nicotinic acetylcholine receptor signalling

Yoichiro Kashiwagi, Manabu Yanagita, Yuko Kojima, Yoshio Shimabukuro, Shinya Murakami
Arch. Oral Biol. May 23, 2012
… methods. [14] and [15] Primer sequences were described previously by Yanagita et al. 15 Human brain RNA (BioChainInstitute Inc., Hayward, CA, USA) was used as positive control. 2.4. Real-time PCR analysis. Isolation of …
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