Molecular cloning and tumour suppressor function analysis of canine REIC/Dkk-3 in mammary gland tumours

Kazuhiko Ochiai, Masami Watanabe, Daigo Azakami, Masaki Michishita, Yasunaga Yoshikawa, Chihiro Udagawa, Pornphimon Metheenukul, Thippayarat Chahomchuen, Hiroshi Aoki, Hiromi Kumon, Masami Morimatsu, Toshinori Omi
Vet. J. May 31, 2013
… RNA was obtained from canine total brain RNA (BioChain) and reverse transcribed using SuperScript III (Life Technologies). PCR amplification was performed using PrimeSTAR (Takara) and dATP was added to the PCR products using a 10× A-attachment kit (Toyobo). …
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