METHOD FOR INDUCING CARDIAC DIFFERENTIATION OF PLURIPOTENT STEM CELL

N Nakatsuji, I Minami, M Uesugi…
US Patent Jul 7, 1905
… Genomic DNA was completely digested with DNase I (Invitrogen … The cycling conditions were
as follows: 10 min at 95° C.; 40 cycles, each consisting of 15 s at 95° C.; 1 min at 60° C. Total
RNA from human adult heart tissue (BioChain) was used to evaluate the expression levels
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