Expression of corticotropin releasing factor receptor type 1 (CRF1) in the human gastrointestinal tract and upregulation in the colonic mucosa in patients with ulcerative colitis

Pu-Qing Yuan, S. Vincent Wu, Julie Elliott, Peter A Anton, Ekaterini Chatzaki, Mulugeta Million, Yvette Taché
Digestive Diseases Research Center Aug 28, 2012
“Total RNA samples extracted from whole thickness GI segments including the esophagus, gastric fundus, pylorus, duodenum, jejunum, ileum, cecum, ascending colon, descending colon, transverse colon, sigmoid colon and rectum and paraffin tissue slides of sigmoid colon were purchased from BioChain Institute, Inc.” “cDNA was synthesized using total RNA extracted from whole thickness segments of healthy subject GI tract.”
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