Administration of IL-33 induces airway hyperresponsiveness and goblet cell hyperplasia in the lungs in the absence of adaptive immune system

Yuichi Kondo, Tomohiro Yoshimoto, Koubun Yasuda, Shizue Futatsugi-Yumikura, Mai Morimoto, Nobuki Hayashi, Tomoaki Hoshino, Jiro Fujimoto, Kenji Nakanishi
Int. Immunol Jun 1, 2008
… …human IL-33 was made by Hokudo Co., Ltd (Sapporo, Japan). Briefly, IL-33 (mature form) was amplified from human lung cDNA (BioChain Institute) as a template and subcloned into pET28a vector (Novagen). BL21 (DE3) RIL was transformed and expressed recombinant… …
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