Visual detection of isothermal nucleic acid amplification using pH-sensitive dyes
NA Tanner, Y Zhang, TC Evans Jr
biotechniques.com Jul 7, 1905
… Tris vs. 400 µM). For RT-LAMP, identical conditions were used with the addition_x000D_
of 0.3 U/µL RTx reverse transcriptase (New England Biolabs) and 10 ng Jurkat_x000D_
total RNA (BioChain, Newark, CA) as substrate. All solutions of …
of 0.3 U/µL RTx reverse transcriptase (New England Biolabs) and 10 ng Jurkat_x000D_
total RNA (BioChain, Newark, CA) as substrate. All solutions of …
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