Ubiquitin C-terminal hydrolase-L5 is required for high glucose-induced transforming growth factor-β receptor I expression and hypertrophy in mesangial cells

Yu-Min Ko, Chun-Ying Chang, Shean-Jaw Chiou, Fu-Jie Hsu, Jau-Shyang Huang, Yu-Lin Yang, Jinn-Yuh Guh, Lea-Yea Chuang
Arch. Biochem. Biophys. Jul 15, 2013
… Briefly, the cells were rinsed twice with ice-cold phosphate-buffered saline, trypsinized, pelleted, and fractionated into cytosolic and nuclear fractions with the CNM compartment protein extraction kit (BioChain Institute, Inc., Hayward, CA) as recommended by the manufacturer. …

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