Transferring the characteristics of naturally occurring and biased antibody repertoires to human antibody libraries by trapping CDRH3 sequences

Sophie Venet, Ulla Ravn, Vanessa Buatois, Franck Gueneau, Sébastien Calloud, Marie Kosco-Vilbois, Nicolas Fischer
PLoS ONE Aug 31, 2012
… Primers were also biotinylated for further purification. Capture of human CDRH3. Human CDRH3 from three donors were amplified by nested PCR from commercially available peripheral blood purified cells cDNA (AllCells and BioChain). …

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