Tissue-specific Distribution and Dynamic Changes of 5-Hydroxymethylcytosine in Mammalian Genomes

Shannon Morey Kinney, Hang Gyeong Chin, Romualdas Vaisvila, Jurate Bitinaite, Yu Zheng, Pierre-Olivier Estève, Suhua Feng, Hume Stroud, Steven E. Jacobsen, Sriharsa Pradhan
J. Biol. Chem. Jul 1, 2011
… …Qiagen DNeasy Blood and Tissue kit. NIH 3T3 and HeLa cells were obtained from ATCC. DNA from human tissues was purchased from Biochain. Five g of genomic DNA (E14 and human normal brain) was digested with MspI (NEB). Digested DNA was purified with phenol chloroform… …

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