The multivalent PDZ domain-containing protein PDZK1 regulates transport activity of renal urate-anion exchanger URAT1 via its C-terminal

Naohiko Anzai, Hiroki Miyazaki, Rie Noshiro, Suparat Khamdang, Arthit Chairoungdua, Ho-Jung Shin, Atsushi Enomoto, Shinichi Sakamoto, Taku Hirata, Kimio Tomita, Yoshikatsu Kanai, Hitoshi Endou
J. Biol. Chem. Oct 1, 2004
… We used human single-tissue slides (Biochain) for light microscopic immunohistochemical analysis using the streptavidin-biotin-HRP complex technique (LSAB kit; DAKO, Carpinteria, CA). …_x000D_
… For the coimmunoprecipitation of endogenous URAT1 and PDZK1, we used human kidney membrane fractions (Biochain) and added 13 anti-PDZK1 antibody or control IgG to this solution. …

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