Role of Cellular Cholesterol Metabolism in Vascular Cell Calcification

Yifan Geng, Jeffrey J. Hsu, Jinxiu Lu, Tabitha C. Ting, Makoto Miyazaki, Linda L. Demer, Yin Tintut
J. Biol. Chem. Sep 1, 2011
… …from cells using TRIzol reagent (Invitrogen). Real-time RT-quantitative PCR (qPCR) was performed using a One-Step qRT-PCR kit (BioChain Institute, Inc. Hayward, CA) in an Mx3005P system (Stratagene La Jolla, CA). beta-Actin was used for normalization. Data Analysis… …

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