Regulation of interleukin-6 expression in osteoblasts by oxidized phospholipids

Wendy Tseng, Jinxiu Lu, Gail A. Bishop, Andrew D. Watson, Andrew P. Sage, Linda Demer, Yin Tintut
J. Lipid Res. May 1, 2010
… …Total RNA was isolated using TRIzol reagent (Invitrogen). Real-time PCR was performed using the One-Step qRT-PCR SuperMix Kit (BioChain, Inc.) and Mx3005P (Stratagene). DNA constructs Generation of murine IL-6 promoter luciferase reporter constructs was previously… …

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