PKA-induced Receptor Activator of NF- B Ligand (RANKL) Expression in Vascular Cells Mediates Osteoclastogenesis but Not Matrix Calcification

Wendy Tseng, Lucia S. Graham, Yifan Geng, Aneela Reddy, Jinxiu Lu, Rita B. Effros, Linda Demer, Yin Tintut
J. Biol. Chem. Sep 1, 2010
… …Total RNA was isolated using TRIzol reagent (Invitrogen). Real-time PCR was performed using the One-Step qRT-PCR SuperMix Kit (BioChain, Inc.) and Mx3005P (Stratagene). Western Analysis Western analysis of whole cell lysates was performed using standard protocols… …

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