Phthalates stimulate the epithelial to mesenchymal transition through an HDAC6-dependent mechanism in human breast epithelial stem cells

Tsung-Hua Hsieh, Cheng-Fang Tsai, Chia-Yi Hsu, Po-Lin Kuo, Jau-Nan Lee, Chee-Yin Chai, Ming-Feng Hou, Chia-Cheng Chang, Cheng-Yu Long, Ying-Chin Ko, Eing-Mei Tsai
Toxicol. Sci. Aug 16, 2012
… Subcellular fractionation. To assess protein translocation, the nuclearand cytoplasmic fractions of R2d cells were isolated using the Compart mental Protein Extraction kit CNMCS (BioChain, Hayward, CA) according to the manufacturer’s protocol. …

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