Overexpression of (His)6-tagged human arginase I in Saccharomyces cerevisiae and enzyme purification using metal affinity chromatography

Andriy E Zakalskiy, Oksana M Zakalska, Yuriy A Rzhepetskyy, Natalia Potocka, Oleh V Stasyk, Daniel Horak, Mykhailo V Gonchar
Protein Expr. Purif. Jan 9, 2012
… The open reading frame of the human arginase I gene (HsARG1) was isolated from human liver cDNA (BioChain Institute, Inc, CA, USA) as a 969 bps long DNA fragment by PCR, using HindIII-flanked primers OL130 (5′-TTGGAAGCTTATGAGCGCCAAGTCCAGA-3′) and …

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