Ouabain activates the Na-K-ATPase signalosome to induce autosomal dominant polycystic kidney disease cell proliferation

Anh-Nguyet T. Nguyen, Kyle Jansson, Gladis Sánchez, Madhulika Sharma, Gail A. Reif, Darren P. Wallace, Gustavo Blanco
Am J Physiol Renal Physiol Oct 1, 2011
… …fraction preparations. Nuclear extracts were prepared for determination of p21 and p27 using the CNM Compartment extraction kit (Biochain Institute, Hayward, CA), following the maufacturer’s instructions. Briefly, ADPKD cells were treated in the absence or presence… …

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