Oncogenic miRNA-182-5p targets Smad4 and RECK in human bladder cancer

Hiroshi Hirata, Koji Ueno, Varahram Shahryari, Yuichiro Tanaka, Z Laura Tabatabai, Yuji Hinoda, Rajvir Dahiya
PLoS ONE Dec 11, 2012
… In order to construct target gene (RECK, Smad4) over expressing plasmids, the genes were amplified with total RNA from human adult normal kidney tissues (catalog#: R1234142-50, BioChain Institute, Newark, CA) and RWPE-1 by transcription–polymerase chain reaction (RT …

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