MicroRNAs 221 and 222 inhibit normal erythropoiesis and erythroleukemic cell growth via kit receptor down-modulation

Nadia Felli, Laura Fontana, Elvira Pelosi, Rosanna Botta, Desirée Bonci, Francesco Facchiano, Francesca Liuzzi, Valentina Lulli, Ornella Morsilli, Simona Santoro, Mauro Valtieri, George Adrian Calin, Chang-Gong Liu, Antonio Sorrentino, Carlo M. Croce, Cesare Peschle
PNAS Dec 1, 2005
… ..Plasmids and Constructs. PGL3-3′ UTR plasmid. Kit 3′ UTR was cloned from human spleen genomic DNA (BioChain, Hayward, CA) by using the forward primer 5′-CTCGAGCGTCTTAGTCCAAACCCAG-3′ and the reverse primer 5′-CTCGAGCAAGGACAAAAGATCT-3…

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