Methods for qPCR gene expression profiling applied to 1440 lymphoblastoid single cells

Kenneth J Livak, Quin F Wills, Alex J Tipping, Krishnalekha Datta, Rowena Mittal, Andrew J Goldson, Darren W Sexton, Chris C Holmes
Methods Jan 14, 2013
… 2.3. Testing of assays with cDNA prepared from bulk RNA. The assays were tested with Universal Human cDNA (BioChainC4234565-R) and with cDNA prepared from bulk total RNA extracted from cell lines GM06991, GM10839, GM12801, and GM12802. …

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