KRAS gene mutations in correlation with clinicopathological features of colorectal carcinomas in Indian patient cohort

Harshali Patil, Rajesh Korde, Arnab Kapat
Med. Oncol Jun 4, 2013
… In total, 25–50 ng of DNA was added to a volume of 2 mM deoxynucleotide triphosphates (BioChain), 10 pmol of each primer, 5U of DNA polymerase (Promega), 0.1 % BSA, 6 % dimethylsulfoxide, 25 mM MgCl2 and 5X polymerase chain reaction (PCR) buffer (Promega). …

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