Impaired trafficking of choline transporter-like protein-1 at plasma membrane and inhibition of choline transport in THP-1 monocyte-derived macrophages

Morgan D. Fullerton, Laura Wagner, Zongfei Yuan, Marica Bakovic
Am J Physiol Cell Physiol Apr 1, 2006
… …30 s at 72C, and a final extension for 10 min at 72C. The CHT1 mRNA in THP-1 cells was compared with human brain cDNA (BioChain) as a positive control using PCR conditions of 94C for 10 min, 40 cycles of 45 s at 94C, 45 s at 50C, and 45 s at 72C… …

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