IGFBP7 is a p53-responsive gene specifically silenced in colorectal cancer with CpG island methylator phenotype

Hiromu Suzuki, Shinichi Igarashi, Masanori Nojima, Reo Maruyama, Eiichiro Yamamoto, Masahiro Kai, Hirofumi Akashi, Yoshiyuki Watanabe, Hiroyuki Yamamoto, Yasushi Sasaki, Fumio Itoh, Kohzoh Imai, Tamotsu Sugai, Lanlan Shen, Jean-Pierre J. Issa, Yasuhisa Shinomura, Takashi Tokino, Minoru Toyota
Carcinogenesis Mar 1, 2010
… …kit (Ambion, Austin, TX). Genomic DNA and total RNA from normal colon tissue from a healthy individual were purchased from BioChain (Hayward, CA). Drug treatment To analyze restoration of IGFBP7 gene expression, CRC cells were treated with 2 muM 5-aza-2-deoxycytidine… …

Leave a Reply