Genomic and Expression Profiling of Glioblastoma Stem Cell–Like Spheroid Cultures Identifies Novel Tumor-Relevant Genes Associated with Survival

Aurélie Ernst, Stefanie Hofmann, Rezvan Ahmadi, Natalia Becker, Andrey Korshunov, Felix Engel, Christian Hartmann, Jörg Felsberg, Michael Sabel, Heike Peterziel, Moritz Durchdewald, Jochen Hess, Sebastian Barbus, Benito Campos, Anna Starzinski-Powitz, Andreas Unterberg, Guido Reifenberger, Peter Lichter, Christel Herold-Mende, Bernhard Radlwimmer
Clin. Cancer Res. Nov 1, 2009
… …candidate genes in tumors, a reference of total RNA obtained from nonneoplastic human brain tissue samples of five individuals (BioChain) was used. Total RNA was treated with DNaseI (Invitrogen) and reverse-transcribed with SuperscriptII (Invitrogen). Each cDNA… …

Leave a Reply